2型糖尿病并发幽门螺杆菌感染胰岛功能及骨质疏松指标变化和TNF-α基因多态性  

Islet function and osteoporosis indexes of type 2 diabetes mellitus patients complicated with Helicobacter pylori infection and TNF-αgene polymorphisms

作　　者：申东雯 肖红珍[1] 俞芳[1] 孟成远 刘俊杰[1] SHEN Dong-wen;XIAO Hong-zhen;YU Fang;MENG Cheng-yuan;LIU Jun-jie(Tangshan Workers Hospital,Tangshan,Hebei 063000,China)

机构地区：[1]唐山工人医院内分泌科,河北唐山063000

出　　处：《中华医院感染学杂志》2024年第9期1326-1330,共5页Chinese Journal of Nosocomiology

基　　金：河北省医学科学研究课题计划(20230235)。

摘　　要：目的探究2型糖尿病合并幽门螺杆菌感染对患者胰岛功能及骨质疏松指标和肿瘤坏死因子-α(TNF-α)基因多态性。方法选取唐山工人医院2022年1月-2023年3月收治的163例2型糖尿病患者,将合并幽门螺杆菌感染和未合并幽门螺杆菌感染分别纳入感染组(95例)和非感染组(68例),分析两组患者胰岛功能及骨质疏松相关指标水平,采用限制性片段长度多态性聚合酶链反应(PCR-RFLP)检测TNF-α基因多态性。结果163例2型糖尿病患者中有95例合并幽门螺杆菌感染,感染率为58.28%;感染组患者高密度脂蛋白胆固醇(HDL-C)水平低于非感染组(P<0.05),总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)水平高于非感染组(P<0.05);与非感染组比较,感染组胰岛功能指标胰岛功能指数(HOMA-IR)、β细胞功能指数(HOMA-β)水平较高(P<0.05),空腹胰岛素(FINS)水平较低(P<0.05);骨质疏松相关指标Ⅰ型原胶原氨基端延长肽(PINP)、骨钙素(OC)、胰岛素样生长因子-1(IGF-1)、Ⅰ型胶原羧基端肽β特殊序列(β-CTX)水平较低(P<0.05);TNF-α基因863位点CC基因型频率、C等位基因频率较低(P<0.05)。结论2型糖尿病患者幽门螺杆菌感染率较高,幽门螺杆菌感染可导致2型糖尿病患者血脂代谢紊乱,促进胰岛素抵抗,且易于并发骨质疏松症,TNF-α基因多态性可能参与2型糖尿病患者幽门螺杆菌感染病情进展。OBJECTIVE To explore the islet function and osteoporosis indexes of type 2 diabetes mellitus patients complicated with Helicobacter pylori infection and tumor necrosis factor-α(TNF-α)gene polymorphisms.METHODS A total of 163 patients with type 2 diabetes mellitus who were treated in Tangshan Workers Hospital from Jan 2022 to Mar 2023 were enrolled in the study and were divided into the infection group with 95 cases and the non-infection group with 68 cases according to the status of complication with H.pylori infection.The pancreas islet function and osteoporosis-related indexes were observed and compared between the two groups of patients,and the TNF-αgene polymorphisms were detected by means of polymerase-chain-reaction-restriction-fragment-length polymorphism(PCR-RFLP).RESULTS Among the 163 patients with type 2 diabetes mellitus,95 were complicated with H.pylori infection,with the infection rate 58.28%.The high-density lipoprotein cholesterol(HDL-C)of the infection group was significantly lower than that of the non-infection group(P<0.05);the levels of total cholesterol(TC),triglyceride(TG)and low-density cholesterol(LDL-C)of the infection group were significantly higher than those of the non-infection group(P<0.05).The levels of pancreas islet function indexes homeostasis model assessment ofβ(HOMA-β)and homeostatic model assessment of insulin resistance(HOMA-IR)of the infection group were significantly higher than those of the non-infection group(P<0.05),while the fasting insulin(FINS)level of the infection group was significantly lower than that of the non-infection group(P<0.05).The levels of osteoporosis-related indexes osteocalcin(OC),special sequence of C-terminal telopeptideβof typeⅠcollagen(β-CTX)and propeptide of typeⅠprocollagen(PINP)of the infection group were significantly lower than those of the non-infection group(P<0.05).The frequencies of CC genotype and C allele at 863 locus of TNF-αgene were significantly lower in the infection group than in the non-infection group(P<0.05).CONCLUS

关 键 词：2型糖尿病 幽门螺杆菌 骨质疏松症 胰岛功能 肿瘤坏死因子-Α 基因多态性 关联 

分 类 号：R587.1[医药卫生—内分泌]