紫草提取物对大鼠支气管哮喘肺部感染的干预效果及作用机制  

Action mechanisms and interventional effect of radices lithospermi extract on rats with bronchial asthma and pulmonary infection

作　　者：李皖豫 王蓓[1] 张珂[3] 林智峰[2] 刘冬[1] LI Wan-yu;WANG Bei;ZHANG Ke;LIN Zhi-feng;LIU Dong(The First Affiliated Hospital of Shihezi University,Shihezi,Xinjiang 832008,China;不详)

机构地区：[1]石河子大学第一附属医院呼吸与危重症医学科,新疆石河子832008 [2]石河子大学第一附属医院肾病内科,新疆石河子832008 [3]石河子大学药学院,新疆石河子832008

出　　处：《中华医院感染学杂志》2024年第10期1447-1452,共6页Chinese Journal of Nosocomiology

基　　金：兵团指导性科技计划项目(2022ZD039);石河子大学科研计划青年创新培育人才项目(CXPY202220)。

摘　　要：目的分析紫草提取物对支气管哮喘肺部感染大鼠的干预效果及作用机制。方法选取50只SPF级SD大鼠,10只大鼠作为对照组,另外40只建立支气管哮喘肺部感染模型,将30只建模成功大鼠分为模型组、药物对照组、紫草提取物组,每组各10只,观察各组大鼠肺组织病理变化,检测抗氧化情况、炎症因子、肺功能、细胞外调节蛋白激酶/p38丝裂原活化蛋白激酶(ERK/p38MAPK)通路相关mRNA及蛋白表达量。结果与对照组相比,模型组、药物对照组、紫草提取物组丙二醛(MDA)、可溶性髓系细胞触发受体-1(sTREM-1)、降钙素原(PCT)、白细胞介素-6(IL-6)水平、ERK/p38MAPK通路相关mRNA及蛋白表达量升高(P<0.05),超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、第1秒用力呼气容积(FEV_(1))、用力肺活量(FVC)、FEV_(1)/FVC水平降低(P<0.05);与模型组相比,药物对照组、紫草提取物组MDA、sTREM-1、PCT、IL-6水平、ERK/p38MAPK通路相关mRNA及蛋白表达量降低(P<0.05),SOD、GSH-Px、FEV_(1)、FVC、FEV_(1)/FVC水平升高(P<0.05);与药物对照组相比,紫草提取物组MDA、sTREM-1、PCT、IL-6水平、ERK/p38MAPK通路相关mRNA及蛋白表达量降低(P<0.05),SOD、GSH-Px、FEV_(1)、FVC、FEV_(1)/FVC水平升高(P<0.05)。结论采用紫草提取物对支气管哮喘肺部感染大鼠干预,可使大鼠抗氧化情况得到改善,并降低大鼠体内炎症因子水平,提高大鼠肺功能,其作用机制与ERK/p38 MAPK信号通路有关。OBJECTIVE To observe the action mechanisms and interventional effect of radices lithospermi extract on the rats with bronchial asthma and pulmonary infection.METHODS Totally 50 SPF grade SD rats were enrolled in the study,10 of which were assigned as the control group,the rest of 40 rats were established the bronchial asthma and pulmonary infection models,30 rats that were successfully established the models were divided into the model group,the drug control group and the radices lithospermi extract group,with 10 rats in each group.The pathological change of lung tissues of the rats were observed.The antioxidant status,inflammatory factors,lung function indexes and expression levels of extracellular signal regulated kinase/p38 Mitogen activated protein kinase(ERK/p38MAPK)pathway-related mRNA and proteins were detected and compared among the groups.RESULTS The levels of malondialdehyde(MDA),soluble triggering receptor expressed on myeloid cell-1(sTREM-1),procalcitonin(PCT)and interleukin-6(IL-6)and the expression levels of ERK/p38MAPK pathway-related mRNA and proteins were higher in the model group,the drug control group and the radices lithospermi extract group than in the control group(P<0.05);the superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),forced expiratory volume in the first second(FEV_(1)),forced vital capacity(FVC)and FEV_(1)/FVC were lower in the model group,the drug control group and the radices lithospermi extract group than in the control group(P<0.05).The levels of MDA,sTREM-1,PCT and IL-6 and the expression levels of ERK/p38MAPK pathway-related mRNA and proteins were lower in the drug control group and the radices lithospermi extract group than in the model group(P<0.05),while the SOD,GSH-Px,FEV_(1),FVC and FEV_(1)/FVC of the drug control group and the radices lithospermi extract group were higher than those of the model group(P<0.05).The levels of MDA,sTREM-1,PCT and IL-6 and the expression levels of ERK/p38MAPK pathway-related mRNA and proteins were lower in the radices lithospermi extr

关 键 词：支气管哮喘肺部感染 紫草提取物 肺功能 炎症因子 

分 类 号：R285.5[医药卫生—中药学]