孕产妇胎膜早破并发生殖道感染与miR-125、GRO-α和MCP-1的关系及其影响因素  

Relationship between premature rupture of fetal membrane complicated with genital tract infection and miR-125,GRO-αand MCP-1 of puerpera and influencing factors

作　　者：李广华 喻佳 朱林英 龚水萍 朱丽娟 LI Guang-hua;YU Jia;ZHU Lin-ying;GONG Shui-ping;ZHU Li-juan(Jiangri Integrated Traditional Chinese and Western Medicine Hospital,Nanchang,Jiangri330003,China)

机构地区：[1]江西省中西医结合医院体检科,江西南昌330003 [2]江西省中西医结合医院妇产科,江西南昌330003 [3]江西省中西医结合医院生殖科,江西南昌330003

出　　处：《中华医院感染学杂志》2024年第10期1539-1542,共4页Chinese Journal of Nosocomiology

基　　金：江西省卫生健康委科研项目(2021JH031410)。

摘　　要：目的探讨孕产妇胎膜早破并发生殖道感染与微小RNA-125(miR-125)、生长调节致癌基因-α(GRO-α)、单核细胞趋化蛋白-1(MCP-1)的关系及其影响因素。方法选取2019年1月-2022年12月江西省中西医结合医院收治的301例胎膜早破孕产妇为研究组,根据孕产妇胎膜早破并发生殖道感染发生情况分为感染组和非感染组,病例数分别为:61例、240例;另选取同期非胎膜早破健康孕妇322名为对照组,采用实时荧光定量PCR法、双抗体夹心酶联免疫吸附法分别检测外周血miR-125、GRO-α、MCP-1水平。采用Logistic分析法分析孕产妇胎膜早破并发生殖道感染的影响因素。结果Logistic回归分析显示,孕周<35周、破膜时间≥12 h、产后出血、合并妊娠期高血压、合并妊娠期糖尿病均是孕产妇胎膜早破并发生殖道感染的影响因素(OR=2.056;2.113;2.004;2.275;1.954,P<0.05);研究组miR-125、GRO-α、MCP-1水平高于对照组(P<0.05),感染组miR-125、GRO-α、MCP-1表达水平高于非感染组(P<0.05)。结论miR-125、GRO-α、MCP-1在孕产妇胎膜早破并发生殖道感染患者中呈高表达;孕周<35周、破膜时间≥12 h、产后出血、合并妊娠期高血压、合并妊娠期糖尿病均是孕产妇胎膜早破并发生殖道感染的影响因素。OBJECTIVE To explore the relationship between premature rupture of membranes(PROM)complicated with genital tract infection and micrornA-125(miR-125),growth-regulating oncogene-α(GRO-α)and monocyte chemotactic protein-1(MCP-1)of the puerpera and analyze the influencing factors.METHODS A total of 301 puerpera with PROM who were treated in Jiangxi Integrated Traditional Chinese and Western Medicine Hospital from Jan 2019 to Dec 2022 were assigned as the study group and were divided into the infection group with 61 cases and the non-infection group with 240 cases according to the status of genital tract infection.Meanwhile,322 healthy pregnant women without PROM were chosen as the control group.The levels of peripheral blood miR-125,GRO-αand MCP-1 were detected by real-time fluorescent quantitative PCR and double antibody sandwich enzyme-linked immunosorbent assay,respectively.Logistic analysis was performed for the influencing factors for the genital tract infection in the PROM puerpera.RESULTS Logistic regression analysis showed that gestational age less than 35 weeks,time of membrane rupture no less than 12 hours,postpartum hemorrhage,complication with gestational hypertension and complication with diabetes mellitus were the influencing factors for the genital tract infection in the PROM puerpera(OR=2.056,2.113,2.004,2.275,1.954,P<0.05).The levels of miR-125,GRO-αand MCP-1 of the study group were significantly higher than those of the control group(P<0.05).The expression levels of miR-125,GRO-αand MCP-1 of the infection group were significantly higher than those of the non-infection group(P<0.05).CONCLUSION The PROM puerpera complicated with genital tract infection show high expressions of miR-125,GRO-αand MCP-1.The gestational age less than 35 weeks,time of membrane rupture no less than 12 hours,postpartum hemorrhage,complication with gestational hypertension and complication with diabetes mellitus are the influencing factors for the genital tract infection in the PROM puerpera.

关 键 词：胎膜早破 生殖道感染 微小RNA-125 生长调节致癌基因-α 单核细胞趋化蛋白-1 影响因素 

分 类 号：R714.433[医药卫生—妇产科学]