下呼吸道流感病毒感染患儿发生重症的危险因素及与CD226基因多态性的关联  

Risk factors for severe condition in children with lower respiratory tract influenza virus infection and association with CD226 gene polymorphisms

作　　者：马忠正 康伟莉 石和丝 张宁宁 殷站茹 闫平 MA Zhong-zheng;KANG Wei-li;SHI He-si;ZHANG Ning-ning;YIN Zhan-ru;YAN Ping(Hengshui People's Hospital,Hengshui,Hebei 053000,China;不详)

机构地区：[1]衡水市人民医院小儿内科,河北衡水053000 [2]衡水卫生学校基础教学部,河北衡水053000

出　　处：《中华医院感染学杂志》2024年第10期1554-1557,共4页Chinese Journal of Nosocomiology

基　　金：河北省科研基金资助项目(2021KL0324001);衡水市重点研发计划项目(2020014051Z)。

摘　　要：目的分析下呼吸道流感病毒感染患儿发生重症的危险因素及与CD226基因多态性的关联。方法选取2020年7月-2023年4月衡水市人民医院收治的116例下呼吸道流感病毒感染患儿为研究组,其中下呼吸道流感病毒感染重症患儿51例为重症组、下呼吸道流感病毒感染轻症患儿65例为轻症组,另选取年龄和性别相匹配的正常健康体检儿童138名为对照组,采用Logistic回归分析法分析下呼吸道流感病毒感染患儿发生重症的危险因素,采用限制性片段长度多态性聚合酶链反应(PCR-RFLP)检测入组研究对象CD226基因多态性。结果年龄小、秋冬季节、合并基础疾病、户外运动<1.5 h/d和挑食或偏食均为下呼吸道流感病毒感染患儿发生重症的独立危险因素(OR=2.210、2.259、2.330、2.079和2.212,P<0.05);研究组CD226基因rs727088位点GG基因型、G等位基因频次高于对照组(P<0.05),且重症组CD226基因rs727088位点GG基因型、G等位基因频次高于轻症组(P<0.05)。结论年龄小、秋冬季节、有基础疾病、户外运动<1.5 h/d、挑食或偏食均为下呼吸道流感病毒感染患儿发生重症的独立危险因素,且CD226基因多态性也与下呼吸道流感病毒感染患儿病情严重程度具有相关性。OBJECTIVE To investigate the risk factors for severe illness condition in the children with lower respiratory tract influenza virus infection and analyze the association with CD226 gene polymorphisms.METHODS A total of 116 children with lower respiratory tract influenza virus infection who were treated in Hengshui People′s Hospital from Jul 2020 to Apr 2023 were assigned as the study group,51 of which had severe lower respiratory tract influenza virus infection were assigned as the severe group,and 65 had mild lower respiratory tract influenza virus infection were assigned as the mild group.Meanwhile,138 healthy children who matched the age and gender and received physical examination were chosen as the control group.Logistic regression analysis was performed for the risk factors for the severe lower respiratory tract influenza virus infection.The CD226 gene polymorphisms of the enrolled research subjects were detected by means of polymerase-chain-reaction-restriction-fragment-length polymorphism(PCR-RFLP).RESULTS The little age,season of autumn and winter,complication with underlying disease,outdoor activities less than 1.5 h/d and dietary bias were the independent risk factors for the severe lower respiratory tract influenza virus infection(OR=2.210,2.259,2.330,2.079 and 2.212,P<0.05).The frequencies of CC genotype and G allele at rs727088 locus of CD226 gene were significantly higher in the study group than in the control group(P<0.05),and the frequencies of GG genotype and G allele at rs727088 locus of CD226 gene were significantly higher in the severe group than in the mild group(P<0.05).CONCLUSION The little age,season of autumn and winter,underlying disease,outdoor activities less than 1.5 h/d and dietary bias are the independent risk factors for the severe lower respiratory tract influenza virus infection.There is also association between the CD226 gene polymorphisms and the severity of lower respiratory tract influenza virus infection in the children.

关 键 词：下呼吸道 重症下呼吸道感染 流感病毒 感染 CD226 危险因素 基因多态性 

分 类 号：R563.1[医药卫生—呼吸系统]