熊果酸通过调控雌激素受体抑制甲状腺癌BCPAP细胞增殖、迁徙、侵袭的机制研究  

Mechanism of Ursolic Acid in Inhibiting Proliferation,Migration and Invasion of Thyroid Cancer BCPAP Cells by Regulating Estrogen Receptors

作　　者：段祥凯 陈红跃[2] DUAN Xiangkai;CHEN Hongyue(The Second Clinical Medical School of Henan University of Chinese Medicine,Zhengzhou Henan 450000,China;Department of Thyroid and Breast Surgery,The Second Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou Henan 450000,China)

机构地区：[1]河南中医药大学第二临床医学院,河南郑州450000 [2]河南中医药大学第二附属医院甲乳外科,河南郑州1450000

出　　处：《新中医》2024年第9期196-201,共6页New Chinese Medicine

基　　金：河南省中医药管理局科研基金项目(2022ZY2020)。

摘　　要：目的:观察熊果酸对雌激素受体ERα、ERβ表达的影响,研究熊果酸抑制甲状腺癌BCPAP细胞增殖、迁徙、侵袭的机制。方法:Westernblot实验检测人正常甲状腺细胞(Nthy-ori3-1细胞)与甲状腺癌BCPAP细胞中ERα、ERβ的蛋白表达,CCK-8实验检测熊果酸对甲状腺癌BCPAP细胞增殖的影响,细胞划痕实验、Transwell实验检测熊果酸对甲状腺癌BCPAP细胞迁徙、侵袭能力的影响。Western blot实验检测熊果酸对甲状腺癌BCPAP细胞雌激素受体ERα、ERβ表达的影响。结果:Nthy-ori3-1细胞与BCPAP细胞比较,ERα的表达水平较低(P<0.05),ERβ的表达水平较高(P<0.05)。不同浓度的熊果酸(4、8、16、32、64μmol/L)对BCPAP细胞增殖具有抑制作用,呈时间和浓度依赖性(P<0.05)。同时间点,B4组(4μmol/L)、B8组(8μmol/L) BCPAP细胞迁移力低于B0组(0μmol/L)(P<0.05)。与B0组比较,B4组、B8组BCPAP细胞侵袭数明显降低(P<0.05)。熊果酸作用24 h后,B4组、B8组ERα表达水平低于B0组(P<0.05),ERβ表达水平高于B0组(P<0.05)。结论:熊果酸可能通过雌激素受体ERα、ERβ降低甲状腺癌BCPAP细胞增殖、迁徙、侵袭的能力。Objective:To observe the effect of ursolic acid on the expressions of estrogen receptors ERαand ERβ,and to study the mechanism of ursolic acid in inhibiting proliferation,migration and invasion of thyroid cancer BCPAP cells.Methods:The protein expressions of ERαand ERβin human normal thyroid cells(Nthy-ori3-1 cells)and thyroid cancer BCPAP cells were detected by Western blot.The effect of ursolic acid on the proliferation of thyroid cancer BCPAP cells Was detected by CCK-8 assay.The effect of ursolic acid on the migration and invasion of thyroid cancer BCPAP cells was detected by cell scratch assay and Transwell assay.The effect of ursolic acid on the expressions of estrogen receptor ERαand ERβin thyroid cancer BCPAP cells Was detected by Western blot.Results:Compared with BCPAP cells,the expression level of ERαwas lower in Nthy-ori3-1 cells(P<0.05),and the expression level of ERβwas relatively high(P<0.05).Ursolic acid at different concentrations(4,8,16,32,64μmol/L)inhibited the proliferation of BCPAP cells in a time-and concentration-dependent manner(P<0.05).The migration of BCPAP cells in the B4 group(4μmol/L)and the B8 group(8μmol/L)was lower than that in the BO group(Oμmol/L)at the same time point(P<0.05).Compared with the BO group,the number of invasive BCPAP cells in the B4 and B8 groups was significantly decreased(P<0.05).After 24 hours of treatment with ursolic acid,the expression level of ERαin the B4 and B8 groups was lower than that in the B0 group(P<0.05),and the expression level of ERβwas higher than that in the B0 group(P<0.05).Conclusion:Ursolic acid may reduce the proliferation,migration and invasion of thyroid cancer BCPAP cells through estrogen receptors ERαand ERβ.

关 键 词：甲状腺癌BCPAP细胞 熊果酸 ERα ERΒ 细胞增殖 细胞迁徙 细胞侵袭 

分 类 号：R285.5[医药卫生—中药学]