基于微流控芯片的循环肿瘤细胞检测系统研究  

Study on Circulating Tumor Cell Detection System Based on Microfluidic Chip

作　　者：邸元帅 罗吉[1] 高大地 杨皓文 DI Yuanshuai;LUO Ji;GAO Dadi;YANG Haowen(Department of Radiation Oncology,Fudan University Shanghai Cancer Center,Department of Oncology,Shanghai Medical College,Fudan University,Shanghai,200032)

机构地区：[1]复旦大学附属肿瘤医院放射治疗中心,复旦大学上海医学院肿瘤学系,上海市200032

出　　处：《中国医疗器械杂志》2024年第3期277-280,共4页Chinese Journal of Medical Instrumentation

摘　　要：目的实现人体外周血液中循环肿瘤细胞(circulating tumor cells,CTCs)的高通量、高检出率的分选富集与检测,为癌症患者提供高效、精准的早期筛查。方法设计一款分选富集与检测集成化的微流控芯片,采用荧光检测系统进行单细胞水平的CTCs检测,得到样本中的CTCs数量。结果使用裂解红细胞后的外周血液样本进行6次实验,在进样速度达到0.2 mL/h时,CTCs能够达到78.6%的最佳检测率,组内相关系数达到0.8以上。结论CTCs检测系统能够实现较高的检出率并具有良好的可靠性,可以为相关领域的临床研究提供可靠的参考。Objective To achieve high throughput and high detection rate of circulating tumor cells(CTCs)in human peripheral blood,and to provide efficient and accurate early screening for cancer patients.Methods A microfluidic chip with the integration of sorting,enrichment and detection was designed,and CTCs at the single cell level were detected by fluorescence detection system to obtain the number of CTCs in samples.Results The peripheral blood samples after lysed red blood cells were used for 6 experiments.When the injection rate reached 0.2 mL/h,CTCs could reach the best detection rate of 78.6%,and the correlation coefficient within the group was above 0.8.Conclusion CTCs detection system can achieve high detection rate and has good reliability,which can provide a reliable reference for clinical research in related fields.

关 键 词：微流控芯片 循环肿瘤细胞 分选富集 荧光检测 早期筛查 

分 类 号：R318[医药卫生—生物医学工程]