An oxygenating colloidal bioink for the engineering of biomimetic tissue constructs  

作　　者：Seol-Ha Jeong Jarno Hiemstra Patrick V.Blokzijl Rebeca Damian-Ferrara Danilo Martins dos Santos Jéssica H.L.da Fonseca Min-Ho Kang Jihyun Kim Dilara Yilmaz-Aykut Mei L.L.Cham-Pérez Jeroen Leijten Su Ryon Shin 

机构地区：[1]Division of Engineering in Medicine,Department of Medicine,Brigham and Women’s Hospital,Harvard Medical School,Boston,MA 02139,USA [2]Leijten Lab,Department of Developmental BioEngineering,Faculty of Science and Technology,Technical Medical Centre,University of Twente,Drienerlolaan 5,7522 NB Enschede,The Netherlands [3]Nanotechnology National Laboratory for Agriculture(LNNA),Embrapa Instrumentação,Sao Carlos,Sao Paulo 13560-970,Brazil [4]Department of Biomedical-Chemical Engineering,The Catholic University of Korea,43 Jibong-ro,Bucheon-si,Gyeonggi-do 14662,Republic of Korea [5]Department of Biotechnology,The Catholic University of Korea,43 Jibong-ro,Bucheon-si,Gyeonggi-do 14662,Republic of Korea

出　　处：《Bio-Design and Manufacturing》2024年第3期240-261,共22页生物设计与制造（英文）

基　　金：funded by the National Insti-tutes of Health(No.R01 AR074234);AHA collaborative award(No.944227);the Gillian Reny Stepping Strong Center for Trauma Inno-vation at Brigham and Women's Hospital.

摘　　要：Ensuring a sufficient oxygen supply is pivotal for the success of bioprinting applications since it fosters tissue integration and natural regeneration.Variation in oxygen concentration among diverse tissues necessitates the precise recreation of tissue-specific oxygen levels in imprinted constructs to support the survival of targeted cells.Although oxygen-releasing biomaterials,such as oxygen-generating microparticles(OMPs),have shown promise for enhancing the oxygen supply of microenvironments in injured tissues,whether this approach is scalable for large tissues and whether tissue-specific bioinks with varying OMP concentrations remain printable remain unknown.This study addresses this critical gap by introducing an innovative class of engineered oxygenated bioinks that combine colloidal-based microgels with OMPs.We report that incorporating nanosized calcium peroxide(nCaO_(2))and manganese oxide nanosheets(nMnO_(2))into hydrophobic polymeric microparticles enables precise modulation of oxygen release while controlling hydrogen peroxide release.Moreover,the fabrication of oxygenating and cytocompatible colloidal gels is achieved using an aqueous two-phase system.This study thoroughly evaluates the fundamental characteristics of the resulting bioink,including its rheological behaviors,printability,shape fidelity,mechanical properties,and oxygen release properties.Moreover,this study demonstrates the macroscopic scalability and cytocompatibility of printed constructs produced via cell-laden oxygenating colloidal bioinks.By showcasing the effectiveness of extrusion-based bioprinting,this study underscores how it can be used to fabricate biomimetic tissues,indicating its potential for new applications.The findings presented here advance the bioprinting field by achieving scalability with both high cell viability and the possibility of mimicking specifically oxygenated tissues.This work thereby offers a promising avenue for the development of functional tissues with enhanced physiological relevance.

关 键 词：3D bioprinting Bioink Colloidal gels Extrusion printing Oxygen-generating microparticle 

分 类 号：R318[医药卫生—生物医学工程]