Development and characterization of 3D-printed electroconductive pHEMA-co-MAA NP-laden hydrogels for tissue engineering  

作　　者：Sara De Nitto Aleksandra Serafin Alexandra Karadimou Achim Schmalenberger John J.EMulvihill Maurice N.Collins 

机构地区：[1]DIBRIS Polytechnic Inter-School Section,University of Genoa,16126 Genoa,Italy [2]School of Engineering,Bernal Institute,University of Limerick,Limerick V94 T9PX,Ireland [3]School of Natural Sciences,Department of Biological Sciences,University of Limerick,Limerick V94 T9PX,Ireland [4]Health Research Institute,University of Limerick,Limerick V94 T9PX,Ireland [5]AMBER,University of Limerick,Limerick V94 T9PX,Ireland

出　　处：《Bio-Design and Manufacturing》2024年第3期262-276,共15页生物设计与制造（英文）

基　　金：research conducted with the financial support of Science Foundation Ireland under the SFI Research Infrastructure Programme (21/RI/9831);the funding provided by the Irish Research Council through the Irish Research Council Enterprise Partnership Scheme with Johnson and Johnson (EPSPG/2020/78)

摘　　要：Tissue engineering(TE)continues to be widely explored as a potential solution to meet critical clinical needs for diseased tissue replacement and tissue regeneration.In this study,we developed a poly(2-hydroxyethyl methacrylate-co-methacrylic acid)(pHEMA-co-MAA)based hydrogel loaded with newly synthesized conductive poly(3,4-ethylene-dioxythiophene)(PEDOT)and polypyrrole(PPy)nanoparticles(NPs),and subsequently processed these hydrogels into tissue engineered constructs via three-dimensional(3D)printing.The presence of the NPs was critical as they altered the rheological properties during printing.However,all samples exhibited suitable shear thinning properties,allowing for the development of an optimized processing window for 3D printing.Samples were 3D printed into pre-determined disk-shaped configurations of 2 and 10 mm in height and diameter,respectively.We observed that the NPs disrupted the gel crosslinking efficiencies,leading to shorter degradation times and compressive mechanical properties ranging between 450 and 550 kPa.The conductivity of the printed hydrogels increased along with the NP concentration to(5.10±0.37)×10^(−7)S/cm.In vitro studies with cortical astrocyte cell cultures demonstrated that exposure to the pHEMA-co-MAA NP hydrogels yielded high cellular viability and proliferation rates.Finally,hydrogel antimicrobial studies with staphylococcus epidermidis bacteria revealed that the developed hydrogels affected bacterial growth.Taken together,these materials show promise for various TE strategies.

关 键 词：Conductive nanoparticles Hydroxyethyl methacrylate(HEMA) Ultraviolet(UV)polymerization 3D printing 

分 类 号：R318.08[医药卫生—生物医学工程] TQ427.26[医药卫生—基础医学] TP391.73[化学工程]