Highly sensitive ratiometric fluorescent fiber matrices for oxygen sensing with micrometer spatial resolution  

作　　者：Giuliana Grasso Valentina Onesto Stefania Forciniti Eliana D’Amone Francesco Colella Lara Pierantoni Valeria Famà Giuseppe Gigli Rui L.Reis J.Miguel Oliveira Loretta L.del Mercato 

机构地区：[1]nstitute of Nanotechnology,National Research Council(CNR-NANOTEC),c/o Campus Ecotekne,via Monteroni,73100 Lecce,Italy [2]Department of Mathematics and Physics“Ennio De Giorgi”,University of Salento,c/o Campus Ecotekne,via Monteroni,73100 Lecce,Italy [3]3B’s Research Group,I3Bs–Research Institute on Biomaterials,Biodegradables and Biomimetics,Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine,University of Minho,AvePark,Zona Industrial da Gandra,4805-017 Barco,Guimarães,Portugal [4]ICVS/3B’s-PT Government Associate Laboratory,4806-909 Braga/Guimarães,Portugal [5]Department of Experimental Medicine,University of Salento,c/o Campus Ecotekne,via Monteroni,73100 Lecce,Italy

出　　处：《Bio-Design and Manufacturing》2024年第3期292-306,共15页生物设计与制造（英文）

基　　金：funding from the European Research Council (ERC) under the European Union’s (EU’s) Horizon 2020 research and innovation program ERC Starting Grant “INTERCELLMED” (No. 759959);the EU’s Horizon 2020 research and innovation program under grant agreement No. 953121 (FLAMIN-GO);the Associazione Italiana per la Ricerca contro il Cancro (AIRC;MFAG-2019;No. 22902);the “Tecnopolo per la medicina di precisione” (Tecno Med Puglia)-Regione Puglia: DGR n.2117 of 21/11/2018, B84I18000540002;the Italian Ministry of Research (MUR) in the framework of the National Recovery and Resilience Plan (NRRP), “NFFA-DI” Grant (n. B53C22004310006), “I-PHOQS” Grant (n. B53C22001750006) and under the complementary actions to the NRRP, “Fit4MedRob” Grant (PNC0000007, n. B53C22006960001), “ANTHEM” Grant (PNC0000003, n. B53C22006710001), funded by Next Generation EU;the PRIN 2022 (2022CRFNCP_PE11_PRIN2022) funded by European Union-Next Generation EU;the financial support provided under the project “HEALTH-UNORTE: Setting-up biobanks and regenerative medicine strategies to boost research in cardiovascular, musculoskeletal, neurological, oncological, immunological, and infectious diseases” (reference NORTE-01-0145FEDER-000039) funded by the Norte Portugal Regional Coordination and Development Commission (CCDR-N) under the NORTE2020 Program;the AIRC Short-term Fellowship program

摘　　要：Oxygen(O_(2))-sensing matrices are promising tools for the live monitoring of extracellular O_(2) consumption levels in long-term cell cultures.In this study,ratiometric O_(2)-sensing membranes were prepared by electrospinning,an easy,low-cost,scalable,and robust method for fabricating nanofibers.Poly(ε-caprolactone)and poly(dimethyl)siloxane polymers were blended with tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II)dichloride,which was used as the O_(2)-sensing probe,and rhodamine B isothiocyanate,which was used as the reference dye.The functionalized scaffolds were morphologically characterized by scanning electron microscopy,and their physicochemical profiles were obtained by Fourier transform infrared spectroscopy,thermogravimetric analysis,and water contact angle measurement.The sensing capabilities were investigated by confocal laser scanning microscopy,performing photobleaching,reversibility,and calibration curve studies toward different dissolved O_(2)(DO)concentrations.Electrospun sensing nanofibers showed a high response to changes in DO concentrations in the physiological-pathological range from 0.5%to 20%and good stability under ratiometric imaging.In addition,the sensing systems were highly biocompatible for cell growth promoting adhesiveness and growth of three cancer cell lines,namely metastatic melanoma cell line SK-MEL2,breast cancer cell line MCF-7,and pancreatic ductal adenocarcinoma cell line Panc-1,thus recreating a suitable biological environment in vitro.These O_(2)-sensing biomaterials can potentially measure alterations in cell metabolism caused by changes in ambient O_(2)content during drug testing/validation and tissue regeneration processes.

关 键 词：ELECTROSPINNING Ruthenium(II)dichloride Oxygen sensors Ratiometric imaging Fluorescence 

分 类 号：R318[医药卫生—生物医学工程]