凝聚层黏接剂用于湿环境下骨黏接及促骨修复的实验研究  

Experimental study on the application of coacervate adhesive to bone bonding and bone repair in a wet environment

作　　者：刘大铭 张扬 杨晓江 杨意鹏 黄鑫怡 邱海洋 姬宇飞 雷伟 LIU Daming;ZHANG Yang;YANG Xiaojiang;YANG Yipeng;HUANG Xinyi;QIU Haiyang;JI Yufei;LEI Wei(Department of Orthopedics,the First Affiliated Hospital of Air Force Medical University,PLA,Xi'an 710032,Shaanxi,China;Department of Orthopedics,General Hospital of Eastern Theater Command,PLA,Nanjing 210002,Jiangsu,China)

机构地区：[1]空军军医大学第一附属医院骨科,西安710032 [2]东部战区总医院骨科,南京210002

出　　处：《医学研究与战创伤救治》2024年第3期232-237,共6页Journal of Medical Research & Combat Trauma Care

基　　金：国家自然科学基金(82172393);陕西省重点研发计划项目(2023-YBSF-146)。

摘　　要：目的使用聚乙烯醇、单宁酸、羟基磷灰石,制备具有湿环境下骨黏接能力及促骨修复能力的新型凝聚层黏接剂并验证其效果。方法以氰基丙烯酸酯黏接剂为对照组,制备两款新型凝聚层黏接剂,使用红外光谱进行表征,并测量3种黏接剂在湿环境下的骨黏接效果。使用CCK-8法和活死染色评估3种黏接剂浸提液对MC3T3-E1细胞的影响。将3种黏接剂植入兔股骨缺损,使用显微CT对其骨修复能力进行体内实验验证。结果成功构建了PVA@TA、PVA@TA@HA两种凝聚层黏接剂,红外光谱分析结果确定了合成的有效性。两种凝聚层黏接剂在湿环境下的水下黏接强度[(70.2±2.1)kPa、(63.3±2.6)kPa]高于氰基丙烯酸酯的[(22.6±3.8)kPa],差异有统计学意义(P<0.01)。CCK-8法及活死染色均证明了PVA@TA、PVA@TA@HA黏接剂浸提液培养条件下MC3T3-E1细胞的增殖情况好于氰基丙烯酸酯黏接剂浸提液。显微CT定量分析证明了PVA@TA@HA黏接剂植入骨缺损后,新生骨生长情况优于其他两组,体现出更优异的促骨修复能力。结论成功构建了以聚乙烯醇、单宁酸、羟基磷灰石为基础的凝聚层黏接剂,相比于传统的氰基丙烯酸酯黏接剂,具有更优异的湿环境下骨黏接性能及促骨修复效果。Objective To use polyvinyl alcohol,tannic acid and hydroxyapatite to develop a new type of coacervate adhesive with the ability of bone bonding and bone repair in wet environment and to verify its effect.Methods Using cyanoacrylate adhesive as control group,two new coacervate adhesive were prepared,characterized by infrared spectroscopy,and the bonding effect of the three adhesives in wet environment was measured.Their effects on MC3T3-E1 cells were evaluated by CCK-8 method and live staining.They were implanted into the rabbit femur defect,and their bone repair ability was verified by microCT in vivo.Results Two kinds of coacervate adhesives,PVA@TA and PVA@TA@HA,were successfully constructed,and the effectiveness of the synthesis was confirmed by infrared spectrum analysis.The underwater bonding strength[(70.2±2.1)kPa,(63.3±2.6)kPa]of the two coacervate adhesives in wet environment was higher than that of cyanoacrylate[(22.6±3.8)kPa],and the difference was statistically significant(P<0.05).The proliferation of MC3T3-E1 cells under the conditions of PVA@TA,PVA@TA@HA adhesive extract was better than that of cyanoacrylate adhesive extract by CCK-8 method and live staining.Quantitative microCT analysis proved that after implantation of PVA@TA@HA adhesive into bone defects,the growth of new bone was better than that of the other two groups,showing better bone repair promoting ability.Conclusion The coacervate adhesive based on polyvinyl alcohol,tannic acid and hydroxyapatite has been successfully constructed.Compared with the traditional cyanoacrylate adhesive,it has better performance of bone bonding and promoting bone repair in wet environment.

关 键 词：凝聚层 湿环境 骨黏接 骨修复 

分 类 号：R68[医药卫生—骨科学]