“通督解郁”针刺在CUMS抑郁模型大鼠前额叶皮质BDNF/TrkB/CREB通路的作用效应  

Impact of Tongdu Jieyu Acupuncture on BDNF/TrkB/CREB Pathwayof Prefrontal Cortex in CUMS Depression Model Rats

作　　者：翟玲 孙佳姿 陈雪妮 张捷 ZHAI Ling;SUN Jiazi;CHEN Xueni;ZHANG Jie(Shanxi University of Chinese Medicine,Jinzhong 030619,China;Shanxi Provincial Hospital of Traditional Chinese Medicine,Taiyuan 030012,China)

机构地区：[1]山西中医药大学,山西晋中030619 [2]山西省中医院,山西太原030012

出　　处：《针灸临床杂志》2024年第5期68-74,共7页Journal of Clinical Acupuncture and Moxibustion

基　　金：山西省中医药管理局科研项目,编号:2019ZYYC036。

摘　　要：目的:探究K252a的作用下,“通督解郁”针刺在慢性温和不可预知应激(CUMS)模型大鼠前额叶皮质BDNF/TrkB/CREB通路的作用机理。方法:64只SPF级SD大鼠随机分为空白组、模型组、氟西汀组、针刺组、模型+DMSO组、模型+K252a组、氟西汀+K252a组和针刺+K252a组,每组8只。造模在CUMS结合孤养法外,针刺组、针刺+K252a组和氟西汀组、氟西汀+K252a组在造模前30 min分别施行手针百会、神庭,电针内关、太冲治疗(频率2 Hz,连续波,电流1 mA,留针30 min)和灌胃治疗;模型+DMSO组和模型+K252a组、氟西汀+K252a组及针刺+K252a组分别在造模前1 h侧脑室注射DMSO溶液和K252a,共21 d。行为学采用体质量、糖水偏好实验以及强迫游泳实验进行评价;免疫组化检测大鼠前额叶组织BDNF、TrkB和CREB阳性细胞平均光密度值IOD;Western blot及PCR法检测大鼠前额叶皮质中相关蛋白及mRNA表达。统计学分析采用LSD检验和非参数检验。结果:与空白组比较,模型组大鼠的体质量差、糖水偏好指数SPI和BDNF、TrkB、CREB的阳性细胞IOD、蛋白及mRNA表达均明显下调,差异具有统计学意义(P<0.01),强迫游泳的不动时间IT明显上调,差异具有统计学意义(P<0.01)。与模型组比较,针刺组、氟西汀组大鼠的体质量差、SPI和BDNF、TrkB、CREB的阳性细胞IOD、蛋白及mRNA表达均明显上调,差异具有统计学意义(P<0.01),IT下调,差异具有统计学意义(P<0.01),模型+K252a组大鼠的体质量差、SPI和BDNF、TrkB、CREB的阳性细胞IOD、蛋白及mRNA表达减少,差异具有统计学意义(P<0.05),IT增加,差异具有统计学意义(P<0.05)。与针刺组比较,针刺+K252a组大鼠的体质量差、SPI和BDNF、TrkB、CREB的阳性细胞IOD、蛋白及mRNA表达均减少,差异具有统计学意义(P<0.05),IT增加,差异具有统计学意义(P<0.05)。结论:“通督解郁”针刺对CUMS模型大鼠的抑郁行为有明显改善作用,其机制可能上调了前额叶皮质上�Objective:To explore the mechanism of the effect of Tongdu Jieyu acupuncture on the BDNF/TrkB/CREB pathway of prefrontal cortex in chronic unpredictable mild stress(CUMS)depression model rats under the action of K252a.Methods:64 SPF SD rats were randomly divided into the control group,the model group,the Fluoxetine group,the acupuncture group,the model+DMSO group,the model+K252a group,the Fluoxetine+K252a group,and the acupuncture+K252a group,with 8 rats in each group.The depression model was established by CUMS combined with isolation.The acupuncture group and the acupuncture+K252a group were intervened by needling Baihui(DU20)and Shenting(DU24),electro-needling Neiguan(PC6)and Taichong(LR3)for 30 min,continuous wave,frequency of 2 Hz,current of 1mA,at the time of 30 min before modeling;the Fluoxetine group and the Fluoxetine+K252a group were also intervened at 30 min before modeling.The model+DMSO group,the model+K252a group,the Fluoxetine+K252a group and the acupuncture+K252a group were injected with DMSO solution and K252a in the lateral ventricle 1 h before modeling for a total of 21 days.Body mass,sugar and water preference experiment and forced swimming experiment were used to evaluate behavior.The integral optical density(IOD)of BDNF,TrkB and CREB positive cells in the prefrontal cortex of the rats was detected by immunohistochemistry.Western blot and PCR were used to detect the expression of related proteins and mRNA in the prefrontal cortex of the rats.LSD test and non-parametric test were used for statistical analysis.Results:Compared with those in the control group,the body mass difference,sugar and water preference index of SPI,IOD value,and protein and mRNA expressions of BDNF,TrkB and CREB were significantly decreased in the model group(P<0.01),and the immobility time(IT)in forced swimming was significantly increased in the model group(P<0.01).Compared with those in the model group,the body mass difference,SPI,IOD,as well as protein and mRNA expressions of BDNF,TrkB and CREB were significantly up-r

关 键 词：慢性温和不可预知应激 抑郁 通督解郁针刺 前额叶 脑源性神经营养因子/酪氨酸蛋白激酶B/环磷腺苷效应元件结合蛋白通路 阻断剂K252a 

分 类 号：R246.6[医药卫生—针灸推拿学]