日粮能量水平对绵羊睾丸发育和相关基因表达的影响  

Effects of dietary energy levels on testis development and the expression of related genes in sheep

作　　者：赵沛迪 杨可 蒋玉奇 黄轲盼 马明宇 李开栋 许辉 李万宏[1,2] ZHAO Pei-di;YANG Ke;JIANG Yu-qi;HUANG Ke-pan;MA Ming-yu;LI Kai-dong;XU Hui;LI Wan-hong(National Demonstration Center for Experimental Grassland Science Education,College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China;State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems,Key Laboratory of Grassland Livestock Industry Innovation,Ministry of Agriculture and Rural Affairs,Lanzhou University,Lanzhou 730020,China;Animal Husbandry Technology Promotion Station of Minqin County,Minqin 733300,China;Minqin Defu Agricultural Technology Co.,Ltd,Minqin 733300,China)

机构地区：[1]兰州大学草业科学国家级实验教学示范中心,兰州大学草地农业科技学院,甘肃兰州730020 [2]兰州大学草种创新与草地农业生态系统全国重点实验室,农业农村部草牧业创新重点实验室,甘肃兰州730020 [3]民勤县畜牧技术推广站,甘肃民勤733300 [4]民勤县德福农业科技有限公司,甘肃民勤733300

出　　处：《草业学报》2024年第6期219-226,共8页Acta Prataculturae Sinica

基　　金：国家重点研发计划(2021YFD1300905);甘肃省重大专项(20ZD7NA004);兰州大学学生创新创业行动计划项目(20210010053)资助。

摘　　要：为探究日粮能量水平对初情期绵羊睾丸发育的影响,选取45只体重[(24.27±0.63) kg]相近的3月龄美利奴公羔,平均分成3组,每组15只,分别饲喂高(10.5 MJ·kg^(-1))、中(10.1 MJ·kg^(-1))、低(9.7 MJ·kg^(-1))3种能量水平日粮。经过104 d的饲养试验后每组随机选择10只羔羊屠宰,测定其睾丸大小、睾丸组织总抗氧化能力、谷胱甘肽过氧化物酶活性和睾酮水平,利用RT-qPCR方法检测与能量代谢和类固醇激素合成相关基因表达情况。结果表明不同能量水平日粮对羔羊体重[(51.73±0.56) kg,(50.69±0.55) kg,(51.51±1.03) kg]、睾丸重量[(253.60±19.69) g,(274.60±14.17) g,(262.40±19.91) g]、睾丸组织的总抗氧化能力[(10.59±1.13) U·mg^(-1)prot,(8.60±1.00) U·mg^(-1)prot,(7.86±1.73) U·mg^(-1)prot]、谷胱甘肽过氧化物酶活性[(0.28±0.08) U·mg^(-1)prot,(0.14±0.03) U·mg^(-1)prot,(0.23±0.03) U·mg^(-1)prot]和睾酮水平[(0.52±0.07) ng·mg^(-1)prot,(0.35±0.05)ng·mg^(-1)prot,(0.44±0.06) ng·mg^(-1)prot]均无显著影响(P>0.05),但是中能量水平组代谢相关基因IGF-1R和IGF-1以及类固醇激素合成相关基因CYP19A1和3βHSD的表达水平显著高于高能量水平组和低能量水平组(P<0.05)。综上所述,初情期羔羊日粮水平对睾丸能量代谢活动存在影响,中等能量水平日粮促进睾丸组织中代谢相关基因IGF-1R和IGF-1以及类固醇激素合成相关基因CYP19A1和3βHSD表达,但对睾丸发育无显著影响。We investigated the effect of dietary energy levels on testicular development in sheep during the pre-puberty stage.Forty-five 3-month-old merino lambs with similar body weight[(24.27±0.63)kg]were randomly divided into three groups;namely high(10.5 MJ·kg^(-1)),medium(10.1 MJ·kg^(-1))and low(9.7 MJ·kg^(-1))energy level groups.After a feeding period of 104 days,10 lambs were randomly selected from each group for slaughter,and their testicular size,total antioxidant levels in testicular tissue,glutathione peroxidase activity,and testosterone levels were measured.The transcript levels of genes related to energy metabolism and steroid hormone synthesis were detected by RT-qPCR.The results indicated that the diets with different energy levels did not significantly affect body weight(51.73±0.56 kg,50.69±0.55 kg and 51.51±1.03 kg for high,medium and low energy level groups,respectively),testicular weight(253.60±19.69 g,274.60±14.17 g and 262.40±19.91 g,respectively),total antioxidant level(10.59±1.13 U·mg^(-1) prot,8.60±1.00 U·mg^(-1) prot and 7.86±1.73 U·mg^(-1) prot,respectively),glutathione peroxidase activity(0.28±0.08 U·mg^(-1) prot,0.14±0.03 U·mg^(-1) prot and 0.23±0.03 U·mg^(-1) prot,respectively),or testosterone level(0.52±0.07 ng·mg^(-1) prot,0.35±0.05 ng·mg^(-1) prot and 0.44±0.06 ng·mg^(-1) prot,respectively)in testicular tissue(P>0.05).However,the transcript levels of the metabolism-related genes IGF-1R and IGF-1 and the steroidogenesis-related genes CYP19A1 and 3βHSD were significantly higher in the middle energy level group than in the other two groups(P<0.05).In summary,dietary energy levels potentially affect testicular metabolic activity in sheep.Feeding lambs with a middle energy level diet during the pre-puberty stage promoted the expression of metabolism-and steroidogenesis-related genes,but did not significantly affect testicular development.

关 键 词：能量水平 繁殖能力 睾丸发育 初情期 

分 类 号：S826.5[农业科学—畜牧学]