牦牛皮下脂肪组织miRNA的鉴定与分析  

Identification and analysis of miRNAs in subcutaneous fat tissue of yaks

作　　者：丁维芹 孙永刚[1,2] 韩银仓 刘亚倩[1,2] 靳生伟 DING Wei-qin;SUN Yong-gang;HAN Yin-cang;LIU Ya-qian;JIN Sheng-wei(Academy of Animal Science and Veterinary Medicine,Qinghai University,Xining 810016,China;Key Laboratory of Plateau Livestock Genetic Resources Protection and Innovative Utilization of Qinghai Provincial,Xining 810016,China)

机构地区：[1]青海大学畜牧兽医科学院,青海西宁810016 [2]青海省高原家畜遗传资源保护与创新利用重点实验室,青海西宁810016

出　　处：《草业学报》2024年第6期227-235,共9页Acta Prataculturae Sinica

基　　金：国家自然科学基金(32060750);青海省科技厅项目(2023-NK-X02,2023-NK-P50)资助。

摘　　要：为鉴定不同饲养方式下牦牛皮下脂肪组织中差异表达的微小RNAs(miRNAs),本试验采用高通量测序对不同饲养方式下牦牛皮下脂肪组织中的miRNAs进行筛选,运用DESeq分别鉴定出自然放牧18月龄(G18) vs (从自然放牧18月龄开始舍饲育肥6个月至24月龄)F24、G18 vs (自然放牧至24月龄)G24和F24 vs G24皮下脂肪组织中差异表达的miRNAs,对差异表达的miRNAs进行靶基因预测分析。在9个牦牛皮下脂肪组织中共鉴定出1158个miRNAs,其中已知miRNAs 731个,新预测miRNAs 427个。在G18 vs F24中有43个差异miRNAs,预测到的靶基因2436个;在G18 vs G24中有68个差异miRNAs,预测到的靶基因3559个,在F24 vs G24中有31个差异miRNAs,预测到的靶基因1456个。对预测到的基因进行GO和KEGG富集分析,其主要富集到了脂肪酸代谢过程、脂肪酸生物合成过程、脂肪酸生物氧化、不饱和脂肪酸代谢过程和脂肪细胞分化调节等。因此,枯草期对牦牛进行补饲有利于牦牛皮下脂肪组织沉积。The aim of this work was to determine how different types of feeding affect fat deposition in yaks.Specifically,we aimed to identify differentially expressed microRNAs(miRNAs)in subcutaneous fat tissues of yaks raised under different feeding methods.High-throughput sequencing data were analyzed to identify the miRNAs present in the subcutaneous adipose tissues of yaks fed using different methods,and DESeq was used to identify the differentially expressed miRNAs among the different feeding methods.The target genes of the differentially expressed miRNAs were predicted using online tools.The treatment groups were F24,G18,and G24.G18 were naturally grazed 18-month-old yaks,G24 were naturally grazed 24-month-old yaks,and F24 were yaks that had been fattened for 6 months since G18.A total of 1158 miRNAs were identified in the subcutaneous fat tissue of nine yaks,including 731 known miRNAs and 427 newly identified miRNAs.There were 43 differentially expressed miRNAs(with 2436 predicted target genes)in the G18 vs F24 comparison;68 differentially expressed miRNAs(with 3559 predicted target genes)in the G18 vs G24 comparison;and 31 differentially expressed miRNAs(with 1456 predicted target genes)in the F24 vs G24 comparison.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses showed that the predicted target genes of the differentially expressed miRNAs were enriched in fatty acid metabolism,fatty acid biosynthesis,fatty acid biooxidation,unsaturated fatty acid metabolism,and regulation of adipocyte differentiation.Therefore,supplemental feeding of yaks during the dry grass period leads to subcutaneous fat tissue deposition.

关 键 词：牦牛 皮下脂肪 MIRNA 靶基因 

分 类 号：S823.85[农业科学—畜牧学]