长期二硫化碳胁迫下硝化污泥微生物菌群变化  

CHANGES IN MICROBIAL COMMUNITY OF NITRIFYING SLUDGE UNDER LONG-TERM CARBON DISULFIDE STRESS

作　　者：周奇 韩培培[1] 侯雅男 黄聪[2] ZHOU Qi;HAN Peipei;HOU Yanan;HUANG Cong(Tianjin University of Science and Technology,Tianjin 300457,China;Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China)

机构地区：[1]天津科技大学,天津300457 [2]中国科学院天津工业生物技术研究中心,天津300308

出　　处：《环境工程》2024年第3期51-57,共7页Environmental Engineering

基　　金：天津市合成生物技术创新能力提升行动--环境与能源安全合成微生物组(TSBICIP-CXRC-007)

摘　　要：我国每年约有近百万吨二硫化碳(CS_(2))用于粘胶纤维的生产。工业上常用曝气手段去除CS_(2),然而一些CS_(2)会继续留存在水中并进入污水处理的硝化单元,影响运行效果。研究发现,短期胁迫下10 mg/L CS_(2)即显著抑制硝化污泥活性,低浓度CS_(2)(10~40 mg/L)会促进硝化污泥代谢活性和抗氧化活性,高浓度(100~200 mg/L)则会产生抑制。在长期CS_(2)(20~200 mg/L)胁迫下硝化反应被完全抑制,长期运行过程中发现硝化污泥表面有白色浑浊物,经液相色谱与XPS分析发现该白色浑浊物为单质硫,推测可能发生了CS_(2)→COS→H_(2)S→S0→SO_(3)^(2-)→SO_(4)^(2-)的同化过程。在16S rRNA分析中也发现了参与硫氧化的Actinobacteria、Sinomonas丰度升高,也验证了这一推测。About one million tons of CS_(2)is used in the production of viscose fiber in China every year.CS_(2)is often removed by aeration in industry.However,some CS_(2)remains in the water and enters the nitrification unit of wastewater treatment,affecting operational performance.It was found that 10 mg/L CS_(2)significantly inhibited nitrification sludge activity under short-term stress,low concentration of CS_(2)(10 to 40 mg/L)could promote the metabolic activity and antioxidant activity of nitrification sludge,and high concentration of CS_(2)(100 to 200 mg/L)could inhibit the activity.The nitrification reaction was completely inhibited under long-term CS_(2)stress(20 to 200 mg/L),and white turbidity was found on the surface of nitrification sludge during long-term operation.The white turbidity was analyzed as simple sulfur by liquid chromatography and XPS,and the assimilation process of CS_(2)→COS→H_(2)S→S0→SO_(3)^(2-)→SO_(4)^(2-)might occur.In 16S rRNA analysis,it was found that the abundance of Actinobacteria and Sinomonas involved in sulfur oxidation has increased,which also verified this hypothesis.

关 键 词：二硫化碳 硝化作用 代谢活性 氧化应激 微生物群落 

分 类 号：X70[环境科学与工程—环境工程]