Control of defensive behavior by the nucleus of Darkschewitsch GABAergic neurons  

作　　者：Huiying Zhao Jinrong Liu Yujin Shao Xiang Feng Binhan Zhao Li Sun Yijun Liu Linghui Zeng Xiao-ming Li Hongbin Yang Shumin Duan Yan-qin Yu 

机构地区：[1]Department of Neurology of Second Affiliated Hospital and School of Brain Science and Brain Medicine,Zhejiang University School of Medicine,Hangzhou 310058,China [2]Nanhu Brain-Computer Interface Institute,Hangzhou 311100,China [3]Liangzhu Laboratory,MOE Frontier Science Center for Brain Science and Brain-Machine Integration,State Key Laboratory of Brain-Machine Intelligence,Zhejiang University,Hangzhou 311121,China [4]NHC and CAMS Key Laboratory of Medical Neurobiology,Zhejiang University,Hangzhou 310058,China [5]Key Laboratory of Novel Targets and Drug Study for Neural Repair of Zhejiang Province,School of Medicine,Hangzhou City University,Hangzhou 310015,China

出　　处：《National Science Review》2024年第4期64-77,共14页国家科学评论（英文版）

基　　金：This work was supported by grants from STI2030-Major Projects(2021ZD0203400 to Y.Q.Y.);the National Natural Science Foundation of China Major Project(T2293733,T2293730,31970939 to Y.Q.Y.,82288101,82090033,U20A6005 to S.D.);the Natural Science Foundation of Zhejiang Province(LZ22H090001 to Y.Q.Y.,LR24C090001 to H.Y.);the Key R&D Program of Zhejiang Province(2022C03034 to Y.Q.Y.);the CAMS Innovation Fund for Medical Sciences(2019-I2M-5-057 to S.D.);the Non-Profit Central Research Institute Fund of the Chinese Academy of Medical Sciences(2023-PT310-01 to Y.Q.Y.).

摘　　要：The nucleus of Darkschewitsch(ND),mainly composed of GABAergic neurons,is widely recognized as a component of the eye-movement controlling system.However,the functional contribution of ND GABAergic neurons(NDGABA)in animal behavior is largely unknown.Here,we show that NDGABA neurons were selectively activated by different types of fear stimuli,such as predator odor and foot shock.Optogenetic and chemogenetic manipulations revealed that NDGABA neurons mediate freezing behavior.Moreover,using circuit-based optogenetic and neuroanatomical tracing methods,we identified an excitatory pathway from the lateral periaqueductal gray(lPAG)to the ND that induces freezing by exciting ND inhibitory outputs to the motor-related gigantocellular reticular nucleus,ventral part(GiV).Together,these findings indicate the NDGABA population as a novel hub for controlling defensive response by relaying fearful information from the lPAG to GiV,a mechanism critical for understanding how the freezing behavior is encoded in the mammalian brain.

关 键 词：nucleus of Darkschewitsch GABAergic population defensive behavior FREEZING neural circuitry 

分 类 号：Q42[生物学—神经生物学]