Therapeutic effects of Lingguizhugan decoction in a rat model of high-fat diet-induced insulin resistance  

作　　者：Xiao-Ming Liu Shi-Qing Yuan Ying Ning Shi-Jia Nie Xu-Qiong Wang Hong-Yi Jia Xiu-Li Zheng 

机构地区：[1]Department of Basic Medicine,School of Basic Medicine Chengdu University of Traditional Chinese Medicine,Chengdu 611137,Sichuan Province,China [2]Department of Clinical Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 610072,Sichuan Province,China

出　　处：《World Journal of Diabetes》2024年第6期1291-1298,共8页世界糖尿病杂志（英文版）（电子版）

基　　金：Supported by the Preresearch Project of the National Natural Science Foundation of China,No.ZRYY1906;the Applied Basic Research Project of the Science and Technology Department of Sichuan Province,No.2021YJ0154;the Talent Research Promotion Plan of Xinglin Scholars of Chengdu University of Traditional Chinese Medicine,No.QNXZ2019035;the Chengdu University of Traditional Chinese Medicine‘Xinglin Scholars'subject talent research promotion Program(young scholars),No.QNXZ2019037.

摘　　要：BACKGROUND Lingguizhugan(LGZG)decoction is a widely used classic Chinese medicine formula that was recently shown to improve high-fat diet(HFD)-induced insulin resistance(IR)in animal studies.AIM To assess the therapeutic effect of LGZG decoction on HFD-induced IR and explore the potential underlying mechanism.METHODS To establish an IR rat model,a 12-wk HFD was administered,followed by a 4-wk treatment with LGZG.The determination of IR status was achieved through the use of biochemical tests and oral glucose tolerance tests.Using a targeted metabolomics platform to analyze changes in serum metabolites,quantitative real-time PCR(qRT-PCR)was used to assess the gene expression of the ribosomal protein S6 kinase beta 1(S6K1).RESULTS In IR rats,LGZG decreased body weight and indices of hepatic steatosis.It effectively controlled blood glucose and food intake while protecting islet cells.Metabolite analysis revealed significant differences between the HFD and HFDLGZG groups.LGZG intervention reduced branched-chain amino acid levels.Levels of IR-related metabolites such as tryptophan,alanine,taurine,and asparagine decreased significantly.IR may be linked to amino acids due to the contemporaneous increase in S6K1 expression,as shown by qRT-PCR.CONCLUSIONS Our study strongly suggests that LGZG decoction reduces HFD-induced IR.LGZG may activate S6K1 via metabolic pathways.These findings lay the groundwork for the potential of LGZG as an IR treatment.

关 键 词：Lingguizhugan decoction High-fat diet-induced insulin resistance Amino acid metabolism S6K1 

分 类 号：R656.61[医药卫生—外科学]