电针对周围神经损伤大鼠相应脊髓节段神经元铁死亡的影响  

Effect of electroacupuncture antiferroptosis on lumbar spinal cord neurons in rats with peripheral nerve injury

作　　者：郝小倩 张振伟 李征 余少校 黄伟妮 周望高 陈泽华 冼绍祥[2] Hao Xiaoqian;Zhang Zhenwei;Li Zheng;Yu Shaoxiao;Huang Weini;Zhou Wanggao;Chen Zehua;Xian Shaoxiang(Department of Hand Surgery,Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine,Shenzhen 518104,China;Department of Orthopedics,the First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou 510080,China)

机构地区：[1]深圳市中西医结合医院手外科,深圳518104 [2]广州中医药大学第一附属医院骨科,广州510080

出　　处：《中华手外科杂志》2024年第2期178-183,共6页Chinese Journal of Hand Surgery

基　　金：广东省中医药局科研项目(20231281)。

摘　　要：目的研究电针对周围神经损伤大鼠相应脊髓节段神经元铁死亡的调控作用。方法将60只SPF级大鼠随机分为假手术组、模型组和电针组,每组20只。除假手术组大鼠以外,其余两组大鼠均建立坐骨神经损伤模型,造模后1 d在"环跳"和"委中"穴进行电针干预,干预28 d后取材。观察大鼠坐骨神经功能改变和腓肠肌湿重恢复率,并采用HE染色观察坐骨神经和L4~L6节段脊髓神经元细胞形态和数量变化。采用PCR和Western Blot观察脊髓组织GPX4的mRNA和蛋白表达情况,采用铁离子浓度试剂盒检测脊髓组织铁离子浓度变化。结果与假手术组相比,造模后大鼠均出现坐骨神经功能下降和腓肠肌湿重恢复率降低。电针干预后,与模型组相比,电针组大鼠坐骨神经功能指数显著升高,腓肠肌湿重恢复率显著增加。与假手术组相比,模型组大鼠脊髓组织出现GPX4 mRNA和蛋白表达降低,铁离子浓度升高;与模型组相比,电针组大鼠脊髓组织GPX4 mRNA和蛋白表达升高,铁离子浓度降低。结论电针干预可以促进坐骨神经损伤大鼠神经功能恢复,同时电针对相应节段的脊髓神经元具有保护作用,作用机制与抗神经元细胞铁死亡有关。Objective To study the regulatory effect of electroacupuncture on ferroptosis of corresponding spinal cord segment neurons in rats with peripheral nerve injury.Methods Sixty SPF grade rats were randomly divided into sham surgery group,model group,and electroacupuncture group,with 20 rats in each group.Except for the sham operated group rats,the other two groups of rats were established models of sciatic nerve injury.One day after modeling,electroacupuncture intervention was performed at the"Huantiao"and"Weizhong"acupoints,and samples were taken after 28 days of intervention.The functional changes of the sciatic nerve and the recovery rate of wet weight of the gastrocnemius muscle in ratswere observed,and the morphological and quantitative changes of neurons in the sciatic nerve and L4 to L6 spinal cord were detected using HE staining.PCR and Western Blot were used to observe the mRNA and protein expression of GPX4 in spinal cord tissue,and iron ion concentration changes in spinal cord tissue were detected using an iron ion concentration kit.Results Compared with the sham surgery group,all rats showed a decrease in sciatic nerve function and a decrease in wet weight recovery rate of the gastrocnemius muscle after modeling.After electroacupuncture intervention,compared with the model group,the sciatic nerve function index and wet weight recovery rate of the gastrocnemius muscle in the electroacupuncture group were significantly increased.Compared with the sham surgery group,the model group showed a decrease in GPX4 mRNA and protein expression in the spinal cord tissue,and an increase in iron ion concentration.Compared with the model group,the expression of GPX4 mRNA and protein in the spinal cord tissue of rats in the electroacupuncture group increased,while the concentration of iron ions decreased.Conclusion Electroacupuncture intervention can promote the recovery of neural function in rats with sciatic nerve injury,and at the same time,electroacupuncture has a protective effect on spinal cord neurons in the corre

关 键 词：周围神经 神经元 电针 铁死亡 

分 类 号：R245[医药卫生—针灸推拿学]