喜树中一株特异产喜树碱内生真菌Aspergillus niger GP3的分离与鉴定  

Isolationand Identification of Camptothecin-Producing Endophytic Fungus Aspergillus Niger GP3 Isolated From Camptotheca acuminata

作　　者：魏巍[1] 高雪霞 徐铖 安昊 屈保玉 顾成波[1] 袁肖寒[2] Wei Wei(Key Laboratory of Forest Plant Ecology of Ministry of Education,Heilongjiang Provincial KeyLaboratory of Ecological Utilization of Forestry-Based Active Substances,Engineering ResearchCenter of Forest Bio-Preparation of Ministry of Education,College of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin Heilongjiang 150040)

机构地区：[1]东北林业大学,化学化工与资源利用学院,森林植物生态学教育部重点实验室,林业生物制剂教育部工程研究中心,黑龙江省林源活性物质生态利用重点实验室,哈尔滨150040 [2]东北农业大学生命科学学院,哈尔滨150030

出　　处：《黑龙江医药》2024年第3期497-501,共5页Heilongjiang Medicine journal

基　　金：“十四五”国家重点研发计划项目子课题“喜树碱类新型医药中间体及其绿色制备技术”(编号:2022YFD2200805-03)。

摘　　要：目的:从喜树内生真菌中筛选出具有产喜树碱潜力的菌株,为获得喜树碱提供一条新的药源途径。方法:从喜树根皮分离内生真菌,综合利用高效液相色谱法(HPLC)以及超高效液相色谱-高分辨质谱(UPLC-Orbitrap-MS/MS)法对内生真菌次生代谢产物有效活性成分进行分析,并通过形态学和分子学对其特异性真菌进行鉴定。结果:内生真菌GP3具有产喜树碱的化学性质,其菌落圆形、黑褐色、中心凸起、边缘呈纤毛状,经ITS序列比对,该菌与Aspergillus niger ITS序列相似性为99%。结论:GP3是一株具有产喜树碱潜力的内生真菌菌株,鉴定为黑曲霉菌(Aspergillus niger)。Objective:Strains with potential to produce camptothecin were screened from endophytic fungi of Camptotheca camptotheca.,with the aim to provide a source of camptothecin.Methods:Endophytic fungus from root bark of Camptotheca acuminata,was used to isolated,active component of endophytic fungi secondary metaboliteswas detected by HPLC and UPLC-Orbitrap-MS/MS.The specific fungi were identified by morphology and molecular analysis.Results:Endophyte GP3 is an endophytic fungus strain with potential to produce camptothecin.The colony is round,dark brown,with a raised center and a ciliated margin.The ITS sequence is 99%similar to the corresponding sequence of Aspergillus niger.Conclusion:A camptothecin-producing endophytic fungus GP3 was isolated from the Camptotheca acuminata and identified as Aspergillus niger.

关 键 词：喜树 内生真菌 分离 鉴定 喜树碱 

分 类 号：R284.1[医药卫生—中药学]