Establishment of acquired tracheoesophageal fistula using a modified magnetic compression technique in rabbits and its postmodeling evaluation  

作　　者：Han Meng Fu-Yao Nan Na Kou Qin-Yan Hong Ming-Sheng Lv Ju-Bo Li Bao-Jie Zhang Hang Zou Lei Li Hong-Wu Wang 

机构地区：[1]Respiratory Disease Center,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100029,China [2]The First Clinical Medical College,Beijing University of Chinese Medicine,Beijing 100700,China [3]Department of Animal Experimental Center,National Center for Cardiovascular,Fuwai Hospital,Beijing 100037,China

出　　处：《World Journal of Gastrointestinal Surgery》2024年第5期1385-1394,共10页世界胃肠外科杂志（英文版）（电子版）

基　　金：Independent Scientific Research Project for Graduate Students of Beijing University of Chinese Medicine(2023),No.ZJKT2023020.

摘　　要：BACKGROUND Previous studies have validated the efficacy of both magnetic compression and surgical techniques in creating rabbit tracheoesophageal fistula(TEF)models.Magnetic compression achieves a 100%success rate but requires more time,while surgery,though less frequently successful,offers rapid model establishment and technical maturity in larger animal models.AIM To determine the optimal approach for rabbit disease modeling and refine the process.METHODS TEF models were created in 12 rabbits using both the modified magnetic compression technique and surgery.Comparisons of the time to model establishment,success rate,food and water intake,weight changes,activity levels,bronchoscopy findings,white blood cell counts,and biopsies were performed.In response to the failures encountered during modified magnetic compression modeling,we increased the sample size to 15 rabbit models and assessed the repeatability and stability of the models,comparing them with the original magnetic compression technique.RESULTS The modified magnetic compression technique achieved a 66.7%success rate,whereas the success rate of the surgery technique was 33.3%.Surviving surgical rabbits might not meet subsequent experimental requirements due to TEF-related inflammation.In the modified magnetic compression group,one rabbit died,possibly due to magnet corrosion,and another died from tracheal magnet obstruction.Similar events occurred during the second round of modified magnetic compression modeling,with one rabbit possibly succumbing to aggravated lung infection.The operation time of the first round of modified magnetic compression was 3.2±0.6 min,which was significantly reduced to 2.1±0.4 min in the second round,compared to both the first round and that of the original technique.CONCLUSION The modified magnetic compression technique exhibits lower stress responses,a simple procedure,a high success rate,and lower modeling costs,making it a more appropriate choice for constructing TEF models in rabbits.

关 键 词：Tracheoesophageal fistula Modified magnetic compression technique Post-modeling evaluation Pneumonia MALNUTRITION 

分 类 号：R657.16[医药卫生—外科学]