Effect of ginsenoside Rg1 on hematopoietic stem cells in treating aplastic anemia in mice via MAPK pathway  

作　　者：Jin-Bo Wang Ming-Wei Du Yan Zheng 

机构地区：[1]Internal Medicine of Traditional Chinese Medicine,Tongde Hospital of Zhejiang Province,Hangzhou 310012,Zhejiang Province,China [2]Institute of Cardiovascular Disease,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China [3]Shanghai Key Laboratory of Molecular Medical Mycology,Second Affiliated Hospital of Naval Medical University,Shanghai 200003,China [4]Shanghai Key Laboratory of Traditional Chinese Clinical Medicine,Shanghai 201203,China [5]Department of Hepatic,The Xixi Hospital of Hangzhou Affiliated to Zhejiang University of Traditional Chinese Medicine,Hangzhou 310023,Zhejiang Province,China

出　　处：《World Journal of Stem Cells》2024年第5期591-603,共13页世界干细胞杂志（英文版）（电子版）

基　　金：Supported by Hangzhou Municipal Bureau of Science and Technology,No.2021WJCY366.

摘　　要：BACKGROUND Aplastic anemia(AA)presents a significant clinical challenge as a life-threatening condition due to failure to produce essential blood cells,with the current the-rapeutic options being notably limited.AIM To assess the therapeutic potential of ginsenoside Rg1 on AA,specifically its protective effects,while elucidating the mechanism at play.METHODS We employed a model of myelosuppression induced by cyclophosphamide(CTX)in C57 mice,followed by administration of ginsenoside Rg1 over 13 d.The invest-igation included examining the bone marrow,thymus and spleen for pathological changes via hematoxylin-eosin staining.Moreover,orbital blood of mice was collected for blood routine examinations.Flow cytometry was employed to identify the impact of ginsenoside Rg1 on cell apoptosis and cycle in the bone marrow of AA mice.Additionally,the study further evaluated cytokine levels with enzyme-linked immunosorbent assay and analyzed the expression of key proteins in the MAPK signaling pathway via western blot.RESULTS Administration of CTX led to significant damage to the bone marrow’s structural integrity and a reduction in hematopoietic cells,establishing a model of AA.Ginsenoside Rg1 successfully reversed hematopoietic dysfunction in AA mice.In comparison to the AA group,ginsenoside Rg1 provided relief by reducing the induction of cell apoptosis and inflammation factors caused by CTX.Furthermore,it helped alleviate the blockade in the cell cycle.Treatment with ginsenoside Rg1 significantly alleviated myelosuppression in mice by inhibiting the MAPK signaling pathway.CONCLUSION This study suggested that ginsenoside Rg1 addresses AA by alleviating myelosuppression,primarily through modulating the MAPK signaling pathway,which paves the way for a novel therapeutic strategy in treating AA,highlighting the potential of ginsenoside Rg1 as a beneficial intervention.

关 键 词：Aplastic anemia Ginsenoside Rg1 MYELOSUPPRESSION MAPK signaling pathway Bone marrow Hematopoietic stem cells 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]