麻荆宣肺颗粒对人冠状病毒229E感染小鼠肺炎模型的治疗作用  

Effect of Majing Xuanfei Granules(麻荆宣肺颗粒)on Human Coronavirus 229E Mouse Pneumonia Model

作　　者：彭勍[1] 孟硕[1] 鲍岩岩[2] 张业昊 徐立[1] 任钧国[1] 崔晓兰[2] 刘建勋[1] PENG Qing;MENG Shuo;BAO Yanyan;ZHANG Yehao;XU Li;REN Junguo;CUI Xiaolan;LIU Jianxun(Institute of Basic Medical Sciences of Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing Key Laboratory of Pharmacology of Chinese Materia Medica,China Australia International R&D Centre of Traditional Chinese Medicine,Beijing 100091,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Science,Beijing 100700,China)

机构地区：[1]中国中医科学院西苑医院基础医学研究所/中药药理北京市重点实验室/国家中澳中医药国际联合研究中心,北京100091 [2]中国中医科学院中药研究所,北京100700

出　　处：《中华中医药学刊》2024年第6期9-13,I0001-I0003,共8页Chinese Archives of Traditional Chinese Medicine

基　　金：中国中医科学院科技创新工程项目(CI2021A04603);北京市科学技术委员会“十病十药”研发专项(Z171100001717007)。

摘　　要：目的 研究麻荆宣肺颗粒对人冠状病毒299E感染小鼠肺炎模型的治疗作用,为其临床应用提供参考。方法 建立人冠状病毒229E感染小鼠模型,感染当天连续给予麻荆宣肺颗粒共4 d。实验第5天动物称重后,眼眶取血,解剖取肺组织并称重,计算肺指数及肺抑制率,实时荧光定量PCR法(Real-time PCR)检测肺组织中病毒载量,流式细胞术检测免疫细胞百分比,酶联免疫法(ELISA)检测肺组织和血清中炎性因子表达量,免疫组化法检测肺组织血管紧张素转化酶2(ACE2)及线粒体组装受体1(MAS1)表达量,组织病理学检测肺组织病变。结果 麻荆宣肺颗粒能显著降低人冠状病毒229E感染小鼠的肺指数(P<0.01),高、中、低剂量的抑制率分别为66.80%、53.20%、45.20%;并显著降低模型动物肺组织中病毒载量(P<0.01);显著升高模型动物外周血淋巴细胞百分比(P<0.05,P<0.01);显著降低模型动物肺组织和血清中炎症因子的含量(P<0.01);显著提高模型动物肺组织ACE2及MAS1的表达(P<0.05,P<0.01);且对支气管及肺泡组织的炎性病变有一定的改善作用。结论 麻荆宣肺颗粒可通过抑制冠状病毒复制、抑制炎症因子释放、调节免疫平衡等途径对人冠状病毒229E肺炎小鼠发挥治疗作用。Objective To study the efficacy of Majing Xuanfei Granules(麻荆宣肺颗粒)on human coronavirus 229E infected mouse model and provide reference for its clinical application.Methods The human coronavirus 229E infected mouse model was used in this study.On the day of infection,Majing Xuanfei Keli Granules were administered for four days.On the fifth day,the whole blood was collected from orbit after measuring the weight.The lung tissue was dissected,and the lung index and inhibition rate were calculated.The coronavirus 229E virus load in lung tissue was detected by Real-time PCR.The percentage of immu-nocytes was detected by flow cytometry.The contents of inflammatory factors in lung tissue and serum were detected by ELISA.The levels of angiotensin converting enzyme 2(ACE2)and mitochondrial assembly receptor 1(MAS1)in lung tissue were detec-ted by immunohistochemical method.Histopathological analysis was performed with lung tissue.Results The result showed that Majing Xuanfei Granules significantly reduced the lung index of human coronavirus 229E infected mouse model(P<0.01),and the inhibition rates were 66.80%,53.20%and 45.20%,respectively.Majing Xuanfei Granules also significantly reduced the lung tissue viral load(P<0.01),increased the peripheral blood lymphocyte percentage(P<0.05,P<0.01),reduced the in-flammatory factors levels in lung tissue and serum(P<0.01),increased the expressions of ACE2 and MAS1 in lung tissue(P<0.05,P<0.01)and improved the bronchial and alveolar tissue inflammatory lesions.Conclusion Majing Xuanfei Granules showed a therapeutic effect on human coronavirus 229E infected mouse model.The mechanism may be related to virus replication inhibition,immunological regulation and inflammatory factor reduction.

关 键 词：麻荆宣肺颗粒 新冠肺炎 人冠状病毒 小鼠模型 治疗作用 

分 类 号：R256.1[医药卫生—中医内科学]