不同剂量舒芬太尼对围术期认知功能紊乱老年大鼠术后认知功能的影响  

Effects of different doses of sufentanil on postoperative cognitive function in aged rats with perioperative cognitive dysfunction

作　　者：李荣[1] 刘飞飞 LI Rong;LIU Feifei(The First Affiliated Hospital of Baotou Medical College,Baotou 014010,China;The Second Affiliated Hospital of Baotou Medical College)

机构地区：[1]包头医学院第一附属医院,内蒙古包头014010 [2]包头医学院第二附属医院

出　　处：《包头医学院学报》2024年第6期49-55,共7页Journal of Baotou Medical College

基　　金：包头医学院科学研究基金扬帆计划项目课题(BYJJ-YF-2018003)。

摘　　要：目的:评价不同剂量舒芬太尼对围术期认知功能紊乱老年大鼠术后认知受损的影响。方法:60只18~22月龄老年大鼠,采用随机数字表法分成6组:对照组、围术期神经认知紊乱(PND)模型组(脾切除)、舒芬太尼A组(脾切除+舒芬太尼1μg·kg^(-1))、舒芬太尼B组(脾切除+舒芬太尼2μg·kg^(-1))、舒芬太尼C组(脾切除+舒芬太尼4μg·kg^(-1)),舒芬太尼D组(脾切除+舒芬太尼8μg·kg^(-1)),每组10只。通过脾切除手术构建SD雄性老年大鼠PND模型。首先利用Morris水迷宫进行连续5 d的空间获得性实验后,于第6天对除了对照组以外的其它各组老年大鼠进行模型构建和(或)术前腹腔注射舒芬太尼干预。所有老年大鼠均于术后的第1、3和7天再对老年大鼠进行空间记忆测试。结果:空间获得性实验会使老年大鼠对于平台位置的记忆随着时间的延长而逐渐加深(P<0.05),大鼠对平台位置获得了有效的记忆,且各组之间没有差异。参考记忆测试结果表明对照组与其它各组术后的空间记忆能力的差异有统计学意义(P<0.05);模型组与舒芬太尼B组、舒芬太尼C组、舒芬太尼D组术后的空间记忆能力的差异有统计学意义(P<0.05);舒芬太尼C组与除了对照组之外其它各组的空间记忆能力的差异有统计学意义(P<0.05)。结论:不同剂量舒芬太尼对老年大鼠术后空间记忆的影响不同,腹腔注射4μg·kg^(-1)舒芬太尼可以明显改善脾切除PND模型大鼠空间记忆能力,改善术后认知功能。Objective:To evaluate the effects of different doses of sufentanil on postoperative cognitive impairment in aged rats with perioperative cognitive dysfunction.Methods:Sixty aged(18-22 months)rats were randomly divided into 6 groups using a random number table method:control group,PND model group(splenectomy),sufentanil group A(splenectomy+sufentanil 1μg·kg^(-1)),sufentanil group B(splenectomy+sufentanil 2μg·kg^(-1)),sufentanil group C(splenectomy+sufentanil 4μg·kg^(-1)),sufentanil group D(splenectomy+sufentanil 8μg·kg^(-1)),with 10 rats in each group.First,the Morris water maze was used to conduct space acquisition experiments for 5 consecutive days of all the groups.On the 6th day,model construction and/or preoperative sufentanil intraperitoneal intervention were performed on all the other groups of aged rats except for the control group.All aged rats were tested for spatial memory on the 1,3 and 7 days after surgery.Results:Space-acquired experiments made the memory of platform position in aged rats gradually deepen with time(P<0.05),and rats gained effective memory of platform position,there was no difference between the groups.The reference memory test results showed that the difference in spatial memory between the control group and each of the other groups was statistically significant(P<0.05).There was significant difference in spatial memory ability between the model group and the sufentanil B group,the sufentanil C group and the sufentanil D group(P<0.05).In addition to the control group,the spatial memory ability of the sufentanil C group was significantly different from that of the other groups(P<0.05).Conclusion:Different doses of sufentanil have different effects on postoperative spatial memory in elderly rats,while intraperitoneal injection of 4μg·kg^(-1) sufentanil can significantly improve the spatial memory ability and postoperative cognitive function of splenectomy PND model rats.

关 键 词：舒芬太尼 不同剂量 认知功能紊乱 围术期 术后 老年大鼠 

分 类 号：R614[医药卫生—麻醉学]