台湾乳杆菌Lt259对非酒精性脂肪性肝病大鼠肝脏保护作用及肠道菌群的影响研究  

Effects of Lactobacillus taiwanensis Lt259 on hepatoprotection and intestinal flora in rats with non-alcoholic fatty liver disease

作　　者：赵锐清 张舒惟 王若诗 林晓颖 徐明超 张素平 陆瑶 杨晶[2,3] 刘丽云 徐建国[1,2,3] Zhao Ruiqing;Zhang Shuwei;Wang Ruoshi;Lin Xiaoying;Xu Mingchao;Zhang Suping;Lu Yao;Yang Jing;Liu Liyun;Xu Jianguo(School of Public Health,Nanjing Medical University,Nanjing 211166,Jiangsu,China;National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Research Units of Discovery of Unknown Bacteria and Function,Chinese Academy of Medical Sciences,Beijing 102206,China;Hebei Key Laboratory of Intractable Pathogens,Shijiazhuang Center for Disease Control and Prevention,Shijiazhuang 050010,Hebei,China)

机构地区：[1]南京医科大学公共卫生学院,江苏南京211166 [2]中国疾病预防控制中心传染病预防控制所,传染病溯源预警与智能决策全国重点实验室,北京102206 [3]中国医学科学院未知细菌发现和功能研究创新单元,北京102206 [4]石家庄市疾病预防控制中心河北省疑难细菌研究重点实验室,河北石家庄050010

出　　处：《疾病监测》2024年第4期457-465,共9页Disease Surveillance

基　　金：中国医学科学院医学与健康科技创新工程(No.2018RU010)。

摘　　要：目的探究台湾乳杆菌Lt259对非酒精性脂肪性肝病(NAFLD)大鼠肝脏的保护作用以及肠道菌群的影响。方法通过溶血、明胶酶活性及抗生素敏感性实验评价Lt259的安全性;通过疏水性、自聚集实验评价其肠道定植能力;通过喂食高脂饲料构建大鼠NAFLD模型,灌胃Lt259菌悬液干预8周后,观察大鼠肝脏的病理变化,检测大鼠血脂水平、血清促炎因子及内毒素水平;采用16S rRNA基因高通量测序检测肠道菌群。结果Lt259无溶血、明胶酶活性,对所测抗生素均敏感,其疏水性与自聚集结果分别为67.00%±5.77%和63.33%±5.77%。Lt259干预改善了NAFLD大鼠肝脏损伤以及肝功水平(P<0.01);显著降低了NAFLD大鼠血脂、促炎因子及内毒素浓度(P<0.05);改善了NAFLD大鼠肠道菌群结构,降低了变形菌门相对丰度(P<0.05),提高了酪氨酸菌属、经黏液真杆菌属、拉克氏梭状芽孢杆菌属及阿克曼菌属等益生菌属丰度,降低了嗜胆菌属等有害菌属丰度。结论台湾乳杆菌Lt259具有改善NAFLD大鼠肝脏病理改变、脂质沉积、炎症损伤的功效,其机制可能与改善肠道菌群紊乱、提升益生菌丰度有关。Objective To investigate the effects of Lactobacillus taiwanensis(Lt259)on the hepatoprotection and intestinal flora in rats with non-alcoholic fatty liver disease(NAFLD).Methods The safety of Lt259 was evaluated by hemolysis,gelatinase activity and antibiotic sensitivity tests.Its intestinal colonization ability was evaluated by hydrophobicity and selfaggregation tests.A NAFLD rat model was constructed by feeding high-fat chow,and after 8 weeks of intervention by gavage with Lt259 bacterial suspension,pathological changes in the liver of the rats were observed,and the lipid level,serum proinflammatory factors and endotoxin levels were detected.And the intestinal flora was detected by high-throughput sequencing of 16S rRNA gene.Results Lt259 had no hemolytic and gelatinase activities,and was sensitive to all antibiotics tested,with hydrophobicity and self-aggregation results of 67.00%±5.77%and 63.33%±5.77%,respectively.Lt259 intervention mitigated the liver injury and improved liver function level in rats with NAFLD(P<0.01).It significantly lowered the concentrations of lipids,pro-inflammatory factors and endotoxin in NAFLD rats(P<0.05),improved the intestinal flora composition of rats with NAFLD,reduced the relative abundance of Proteobacteria(P<0.05),increased the abundances of Tyzzerella,Blautia,Lachnoclostridium and Akkermansia and other probiotics,and reduced the abundances of Bilophila and other harmful genera.Conclusion L.taiwanensis Lt259 has the efficacy to improve the pathological changes,lipid deposition,and inflammatory injury in the liver of rats with NAFLD,and the mechanism might be related to the reduction of intestinal flora disorders and the enhancement of probiotic abundance.

关 键 词：台湾乳杆菌 肠道菌群 非酒精性脂肪性肝病 降脂 护肝 

分 类 号：R211[医药卫生—中医学] R378.99R589