狭窄通道红细胞跨膜传质特性数值分析  

NUMERICAL ANALYSIS OF TRANSMEMBRANE MASS TRANSFER CHARACTERISTICS OF RED BLOOD CELLS IN NARROW CHANNELS

作　　者：傅尧 王骁龙 龚晓波[1] Fu Yao;Wang Xiaolong;Gong Xiaobo(Department of Engineering Mechanics,School of Naval Architecture,Ocean and Civil Engineering,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海交通大学船舶海洋与建筑工程学院工程力学系,上海200240

出　　处：《力学学报》2024年第5期1297-1306,共10页Chinese Journal of Theoretical and Applied Mechanics

基　　金：国家自然科学基金(120721982);上海交通大学医工交叉基金(YG2022ZD004)资助项目。

摘　　要：随着对血液微流动过程的不断探索,红细胞在微通道中的运动微观特性备受关注,利用流体力学特性的低损伤手段调控细胞体积并完成跨膜传药也逐渐成为研究热点.为了分析红细胞在微通道中的传质和力学特性及其影响因素,采用浸入式边界法研究细胞穿越狭窄通道的整个运动过程,并考虑细胞膜表面透水传质效应,完成细胞跨膜传质过程的数值建模,实现对狭窄通道中红细胞跨膜传质过程微观的传质特性数值分析.研究表明,细胞在挤入、排出狭缝的运动过程中,体积先损失、后恢复,在这个过程中,细胞头部和尾部以向外的渗透流出为主,中间部位则以向内的渗透流入为主.文章的研究进一步表明,狭缝挤压过程中的体积净变化是由向内和向外渗透速度的大小差异引起的,而与向内和向外渗透的膜面积差异并无明显关系.对体积变化的影响因素探究后发现,更狭窄的通道和更大的入口出口角均会引起细胞发生更多的体积损失和体积恢复,且出口角的变化会引起细胞体积恢复后所达到的体积稳定值:在固定入口角时,狭缝出口角越大,细胞离开狭缝后的稳定体积越小;细胞的硬度越大、细胞膜通透性(渗透系数)越大,细胞在狭缝中运动过程中的体积损失和体积恢复程度越高.With the continuous exploration of blood microfluidic processes,the microscopic characteristics of red blood cells(RBCs)in microchannels have attracted attention.Using low-damage methods based on fluid mechanics to regulate cell volume and complete transmembrane drug transfer has gradually become a research hotspot.To further explore the mass transfer and mechanical properties and their influencing factors for RBCs in microchannels,this paper adopts the immersion boundary method to study the entire movement process of cells crossing narrow channels.By supplementing the osmotic slip velocity term on the surface of the cell membrane,the mass transfer process is coupled with cell movement,achieving numerical analysis of the microscopic mass transfer characteristics of red blood cells in narrow channels.The result shows that during the process of cells into and out of the slit,the volume undergoes a process of first loss and then recovery.In this process,the head and tail of the cell mainly penetrate outward,while the middle part mainly infiltrates inward.Furtherly,research suggests that the change in cell volume caused by the difference between inward and outward permeation rate dominantly,not the difference between the membrane area of inward and outward permeation.After exploring the influencing factors of volume changes,we found that narrow channels and larger inlet and outlet angles can cause more volume loss and recovery of cells.When other factors remain unchanged,the larger the outlet angle,the smaller the stable volume of cells after leaving the slit.Additionally,the more the hardness of the cell and the greater the permeability(permeability coefficient)of the cell membrane,the higher the volume loss and recovery degree of the cell during movement in the slit.

关 键 词：红细胞 数值模拟 跨膜传质 浸入式边界法 流固相互作用 

分 类 号：Q66[生物学—生物物理学]