基于非牛顿微液滴的粒子封装及检测  

PARTICLE ENCAPSULATION AND DETECTION BASED ON NON-NEWTONIAN MICRODROPLETS

作　　者：梁定新 吕鑫钰 覃开蓉[2] 薛春东 Liang Dingxin;Lyu Xinyu;Qin Kairong;Xue Chundong(School of Optoelectronic Engineering and Instrumentation Science,Dalian University of Technology,Dalian 116024,Liaoning,China;School of Biomedical Engineering,Dalian University of Technology,Dalian 116024,Liaoning,China)

机构地区：[1]大连理工大学光电工程与仪器科学学院,辽宁大连116024 [2]大连理工大学医学部生物医学工程学院,辽宁大连116024

出　　处：《力学学报》2024年第5期1307-1316,共10页Chinese Journal of Theoretical and Applied Mechanics

基　　金：辽宁省自然科学基金(2021-MS-133);国家自然科学基金(12172081)资助项目。

摘　　要：微流控液滴封装技术可将单个或者多个颗粒物封装到微尺度液滴,具有细胞培养、药物可控释放和微量成分分析等重要生物医学应用,而这些应用往往涉及多相混合的复杂非牛顿流体.目前制备尺寸均匀的非牛顿微液滴并实现高效率单粒子封装仍较难实现.针对该问题,首先基于流动聚焦微通道和聚合物溶液开展非牛顿液滴生成实验,系统探究不同非牛顿性质对液滴生成模态的影响,指出兼具剪切稀化与弹性效应的聚合物溶液可在射流模态下实现高单分散性液滴的稳定生成.在此基础上,结合惯性-黏弹性粒子排序,实现了封装率超过58%的单粒子封装,突破了传统单粒子封装的泊松限制.最后,进一步构建了粒子封装率自动检测模型,验证了其在单液滴与多液滴场景下粒子封装率高精度检测的有效性.综上,研究结果不仅一定程度上拓展对于液滴微流控基础理论的认识,还充分验证了射流模态下非牛顿液滴稳定生成用于单粒子封装策略的可行性和优越性,可为优化基于非牛顿微液滴的粒子封装技术及开发一体化装置提供一定参考.Microfluidic droplet encapsulation technology can encapsulate single particle or multiple particles into microscale droplets.It has important biomedical applications such as cell culture,controlled drug release,and trace component analysis,and most of these practical applications involve complex non-Newtonian fluids with multiple phases.At present,it is still difficult to prepare non-Newtonian microdroplets with uniform size and achieve highefficiency single particle encapsulation with these non-Newtonian microdroplets.In order to address this problem,we first conducted the experiments of non-Newtonian droplet generation and particle encapsulation based on flow-focusing microchannel and polymer solution,and systematically explored the effects of different non-Newtonian properties on droplet generation modes.It was pointed out that the polymer solutions with both shear thinning and elastic effects can realize stable generation of highly monodisperse droplets in jetting mode.On this basis,we combined this stable generation of highly monodisperse droplets in jetting mode with the inertia-viscoelastic particle sorting,and achieved high-efficiency single-particle encapsulation that overcomes the Poisson limitation of single particle encapsulation using traditional methods.Finally,we constructed an automatic detection model of particle encapsulation rate to achieve highprecision detection of the encapsulated particles in both single droplet and multi-droplet scenarios.To sum up,the research results of this paper not only expand the understanding of the basic theory of droplet microfluidic technology to a certain extent,but also fully verify the feasibility and superiority of the strategy of stable generation of non-Newtonian droplet in jetting mode for single-particle encapsulation,which can provide certain reference for optimizing the particle encapsulation technology based on non-Newtonian microdroplet and developing the corresponding integrated device.

关 键 词：非牛顿微液滴 粒子封装 流动聚焦微通道 液滴生成模态 高精度检测 

分 类 号：O35[理学—流体力学]