风湿性心脏病患者外周血miR-23、NF-κB、TGF-β1表达与心功能分级的关系分析  

Relationship between Peripheral Blood miR⁃23,NF⁃κB,TGF⁃β1 and Cardiac Function Grading in Patients with Rheumatic Heart Disease

作　　者：杨亚兵 郑晓丽 孙绪志 王文英 万军[2] YANG Yabing;ZHENG Xiaoli;SUN Xuzhi;WANG Wenying;WAN Jun(Department of Cardiology,Beijing Renhe Hospital,Beijing 100026,China;Department of Cardiology,The Third Affiliated Hospital of Xi′an Jiaotong University,Xi′an 710068,Shaanxi,China)

机构地区：[1]北京仁和医院心内科,北京100026 [2]西安交通大学第三附属医院心内科,西安710068

出　　处：《中国分子心脏病学杂志》2024年第2期6005-6009,共5页Molecular Cardiology of China

基　　金：中国药学会科技开发中心专题研究(CMAI-2021-10-002)。

摘　　要：目的探讨风湿性心脏病(RHD)患者外周血小核糖核酸-23(miR-23)、核因子κB(NF-κB)、转化因子-β1(TGF-β1)表达与心功能分级的关系。方法选取2019年1月至2022年1月确诊为RHD的74例患者纳入RHD组,选择同期门诊体检健康者74例纳入对照组。采用酶联免疫法检测外周血TGF-β1、NF-κB水平,荧光定量PCR检测外周血miR-23的表达水平,超声心动图检查心功能指标。Spearman相关性检外周血miR-23、NF-κB、TGF-β1表达与RHD患者纽约心脏协会(NYHA)心功能分级的关系。结果RHD组外周血miR-23、NF-κB水平显著高于对照组,LVEF及TGF-β1水平显著低于对照组(P均<0.05)。不同NYHA分级RHD患者外周血miR-23、NF-κB、TGF-β1表达水平差异显著(P均<0.05)。外周血miR-23、NF-κB与RHD患者NYHA分级程度呈负相关,TGF-β1与RHD患者NYHA分级呈正相关(P均<0.05)。结论RHD患者外周血NF-κB水平升高,TGF-β1水平降低,其表达水平与心功能分级密切相关。Objective To explore the relationship between peripheral blood microRNA⁃23(miR⁃23),nuclear factor kappa B(NF⁃κB),transforming growth factor⁃β1(TGF⁃β1)and cardiac function grading in patients with rheumatic heart disease(RHD).Methods A total of 74 patients with RHD and 74 healthy controls were enrolled as RHD group and control group between January 2019 and January 2022.The levels of TGF⁃β1 and NF⁃κB in peripheral blood were detected by enzyme⁃linked immunosorbent assay,expression of peripheral blood miR⁃23 was detected by fluorescence quantitative PCR,and cardiac function indexes were examined by echocardiography.The relationship between peripheral blood miR⁃23,NF⁃κB,TGF⁃β1 and grading of New York Heart Association(NYHA)was analyzed by Spearman correlation analysis.Results The levels of peripheral blood miR⁃23 and NF⁃κB in RHD group were significantly higher than those in control group,while LVEF and TGF⁃β1 were significantly lower than those in control group(all P<0.05).There were significant differences in expression levels of miR⁃23,NF⁃κB and TGF⁃β1 among RHD patients with different NYHA grading(all P<0.05).The peripheral blood miR⁃23 and NF⁃κB were negatively correlated with NYHA grading,while TGF⁃β1 was positively correlated with NYHA grading(all P<0.05).Conclusion The level of peripheral blood NF⁃κB is increased,while TGF⁃β1 level is decreased in patients with RHD,and the two indexes are closely related to cardiac function grading.

关 键 词：风湿性心脏病 外周血核因子κB 转化因子-β1 左室射血分数 心功能分级 

分 类 号：R54[医药卫生—心血管疾病]