烙灸对兔激素性股骨头坏死组织中IKKβ、IκBα表达的影响  

Effect of Moxibustion on the Expression of Ikkβ and IκBα in the Femoral Head of Steroid-induced Osteonecrosis in Rabbits

作　　者：徐玉娟 刘园园[1] 陶莹 陈坤 陈梦莹 连佳伟 朱宁[3] XU Yujuan;LIU Yuanyuan;TAO Ying;CHEN Kun;CHEN Mengying;LIAN Jiawei;ZHU Ning(Ningxia Medical University,Yinchuan 750004,China;Ningxia Hui Autonomous Region Ningdong Hospital,Yinchuan 750411,China;General Hospital of Ningxia Medical University,First Clinical Medical College of Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学,银川750004 [2]宁夏回族自治区宁东医院,银川750411 [3]宁夏医科大学总医院,宁夏医科大学第一临床医学院,银川750004

出　　处：《宁夏医科大学学报》2024年第5期440-445,452,共7页Journal of Ningxia Medical University

基　　金：国家自然科学基金项目(81760904);宁夏医科大学总医院“国家自然科学基金项目孵育计划”。

摘　　要：目的探讨烙灸治疗早期激素性股骨头坏死(steroid-induced osteonecrosis of the femoral head,SONFH)的作用机制。方法将新西兰兔按随机数表法分为空白组、模型组、烙灸组、抑制剂组,每组6只,采用内毒素联合激素的方法制备SONFH模型。烙灸组进行4周的烙灸治疗,抑制剂组给予IκBα磷酸化抑制剂Bay11-7082(1 mg·kg-1)腹腔注射3周。干预前后分别观察实验兔一般状况变化;HE染色观察各组实验兔股骨头组织病理学变化;Western blot和RT-qPCR分别检测股骨头组织中IKKβ、磷酸化IκBα(p-IκBα)蛋白及mRNA的表达变化;ELISA检测血清中核因子κB(nuclear factor kappa-B,NF-κB)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)水平。结果与空白组比较,模型组实验兔逐渐出现精神萎靡、毛发暗淡等情况,股骨头组织骨小梁出现断裂、稀疏等改变,IKKβ、p-IκBα蛋白水平,IKKβmRNA水平及血清中NF-κB、TNF-α、IL-6水平均升高(P均<0.05);与模型组比较,烙灸组股骨头组织病理学改善,IKKβ、p-IκBα蛋白水平,IKKβmRNA水平及血清中NF-κB、TNF-α、IL-6水平均降低(P均<0.05),而IκBα蛋白及mRNA表达均升高(P均<0.05)。结论烙灸可改善早期SONFH骨代谢,缓解骨坏死,其机制与抑制TLR4/NF-κB通路有关。Objective To explore the mechanism of moxibustion in the treatment of experimental rabbits with steroid-induced osteonecrosis of the femoral head(SONFH)at the early stage.Methods New Zealand rabbits were randomly divided into blank group,model group,moxibustion group and inhibitor group,with 6 rabbits in each group.The SONFH model was established by the endotoxin united glucocorticoid method.The rabbits in the moxibustion group were treated with moxibustion for 4 weeks,and those in the inhibitor group were treated with intraperitoneal injection of the inhibitor of IκBαphosphorylation Bay11-7082(1 mg·kg-1)for 3 weeks.Before and after intervention,the general condition of experimental rabbits were observed.The changes of femoral head histopathology were observed by HE stain method.Western blot and RT-qPCR were used to detect the protein and mRNA expression of IKKβ,p-IκBαin the femoral head.The levels of NF-κB,TNF-αand IL-6 in serum were detected by ELISA.Results Compared with the blank group,the experimental rabbits in the model group gradually appeared listlessness and dark hair,and the trabecular bone of femoral head appeared fracture and thinning.The expression of IKKβ,p-IκBαprotein,IKKβmRNA and the levels of NF-κB、TNF-α、IL-6 in serum were increased(P all<0.05).Compared with the model group,the histomorphology of the femoral head was improved,the expression of IKKβ,p-IκBαprotein,IKKβmRNA and the levels of NF-κB,TNF-αand IL-6 in the serum were decreased in the moxibustion group(P all<0.05),and the protein and mRNA expression of IκBαincreased(P all<0.05).Conclusion Moxibustion treatment for SONFH can improve bone metabolism in SONFH,alleviate bone necrosis,which may be related to inhibiting TLR4/NF-κB pathway.

关 键 词：烙灸 激素性股骨头坏死 TLR4/NF-κB通路 IKKΒ IΚBΑ 

分 类 号：R285.5[医药卫生—中药学]