枸杞多糖对C57BL/6J骨质疏松症小鼠的早期防治  

Early Prevention and Treatment of Lycium Barbarum Polysaccharideon C57BL/6J Osteoporosis Mice

作　　者：刘艺洁 赵薇[1] 李林隆 李海林 姚建花 李智东 刘飞[2] 丁玉梅[2] 孟海伟 于洋 王银[1] 苗珍花[1] LIU Yijie;ZHAO Wei;LI Linlong;LI Hailin;YAO Jianhua;LI Zhidong;LIU Fei;DING Yumei;MENG Haiwei;YU Yang;WANG Yin;MIAO Zhenhua(Ningxia Medical University,Yinchuan 750004,China;General Hospital of Ningxia Medical University,the First Clinical College of Ningxia Medical University,Yinchuan 750004,China;School of Basic Medicine,Shandong University,Qingdao 250012,China)

机构地区：[1]宁夏医科大学,银川750004 [2]宁夏医科大学总医院,宁夏医科大学第一临床医学院,银川750004 [3]山东大学基础医学院,青岛250012

出　　处：《宁夏医科大学学报》2024年第6期586-591,共6页Journal of Ningxia Medical University

基　　金：国家自然科学基金项目(81060034,81460220);宁夏回族自治区基金项目(2022AAC03610);大学生创新创业训练计划项目(S202310752014)。

摘　　要：目的探讨枸杞多糖(LBP)对C57BL/6J骨质疏松症小鼠的早期防治作用。方法选用12周C57BL/6J雌鼠,采用双侧卵巢完全切除术制备骨质疏松症模型,假手术组切除卵巢周围脂肪组织,术后1周,选取手术成功的小鼠分为假手术组、模型组和LBP组。LBP组给予灌胃LBP 0.1 g·kg^(-1),1次/d,连续12周,假手术组和模型组灌胃等量蒸馏水。酶联免疫吸附(ELISA)法检测小鼠血清中雌二醇(E_(2))含量;生物化学方法检测血清氧化应激指标丙二醛(MDA)、超氧化物歧化酶(SOD)含量;Micro CT扫描胫骨微观结构,观察骨小梁厚度(Tb·Th)、骨表面积和骨体积之比(BS/BV)、骨小梁分离度(Tb·Sp)、骨密度(BMD)、骨体积分数(BV/TV)、骨体积(BV)的变化。结果与假手术组小鼠比较,模型组小鼠的体质量增长率升高、子宫脏器系数明显减小、血清E_(2)含量下降、血清氧化应激指标MDA含量增高、SOD活力降低(P均<0.01);与模型组小鼠比较,LBP组小鼠子宫脏器系数、血清E_(2)含量差异均无统计学意义(P均>0.05),但体质量增长率下降、血清MDA含量降低、SOD活力增加(P均<0.01),骨组织BMD、BV升高(P均<0.05),Tb·Th及BV/TV均显著升高(P均<0.01)。胫骨三维重建图显示,模型组骨皮质变薄,呈现骨质疏松改变,骨小梁数量减少,排列紊乱,髓腔明显扩大;LBP组较模型组骨小梁数量增加。结论LBP可能通过调控早期骨质疏松症小鼠的氧化应激反应,发挥改善骨质疏松的作用。Objective To discuss the early prevention and treatment effect of Lycium barbarum polysaccharide(LBP)on C57BL/6J osteoporosis mice.Methods 12 weeks C57BL/6J female mice were selected to make osteoporosis model by bilateral ovariectomy.In the sham group,fat tissue around the ovaries was removed,then the successful model mice were divided into model group and LBP group 1 week after surgery,LBP group was given LBP 0.1 g·kg^(-1),once a day for 12 weeks,sham group and model group were given the same amount of distilled water.The content of estradiol(E_(2))in serum of mice was detected by ELISA.Serum oxidative stress indexes malondialdehyde(MDA)and superoxide dismutase(SOD)were detected by biochemical methods.The Micro CT scan of tibia was performed to observe the changes of trabecular thickness(Tb·Th),ratio of bone surface area to bone volume(BS/BV),trabecular separation(Tb·Sp),bone density(BMD),bone volume fraction(BV/TV)and bone volume(BV).Results Compared with sham group,the growth rate of body mass in model group was increased,uterine coefficient,serum E_(2)content were decreased,serum oxidative stress index MDA content were increased,SOD activity were decreased(P all<0.01).Compared with model group,there was no significant difference in uterine coefficient and serum E_(2)content in LBP group(P all>0.05),but the growth rate of body mass,serum MDA content were decreased,SOD activity was increased(P all<0.01),bone BMD and BV were increased(P all<0.05),Tb·Th and BV/TV were significantly increased(P all<0.01).The three dimensional reconstruction of tibia showed that the cortex bone in the model group was thin,osteoporosis was present,the number of trabeculae bone was reduced,the arrangement was disordered,and the pulp cavity was obviously enlarged.The number of trabeculae bone in LBP group was higher than that in model group.Conclusion LBP may play a role in ameliorating osteoporosis by regulating oxidative stress response in early osteoporosis mice.

关 键 词：枸杞多糖 C57BL/6J小鼠 骨质疏松症 氧化应激 骨密度 

分 类 号：R285.5[医药卫生—中药学]