桥本甲状腺炎病儿血清肿瘤坏死因子受体相关因子6和神经生长因子受体水平与免疫水平及甲状腺功能的相关性  

Correlation of serum TRAF6 and NGFR levels with immune levels and thyroid function in children with Hashimoto´s thyroiditis

作　　者：崔向明 左雪梅 张海波 李阳 CUI Xiangming;ZUO Xuemei;ZHANG Haibo;LI Yang(Department of Pediatrics,Chengde Maternal and Child Health Hospital,Chengde,Hebei 067055,China)

机构地区：[1]承德市妇幼保健院儿科,河北承德067055

出　　处：《安徽医药》2024年第7期1413-1417,共5页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探究桥本甲状腺炎(HT)病儿血清肿瘤坏死因子受体相关因子6(TRAF6)和神经生长因子受体(NGFR)水平与免疫水平及甲状腺功能的相关性。方法选取2021年3月至2022年3月在承德市妇幼保健院就诊的HT病儿104例作为HT组,根据甲状腺功能分为HT亢进组30例,HT正常组38例,HT减退组36例。采用随机数字表法选取同期在儿科门诊体检健康的儿童95例作为对照组。采用酶联免疫吸附测定(ELISA)检测血清辅助型T细胞1(Th1)因子中的白细胞介素2(IL-2)、γ-干扰素(IFN-γ)、辅助型T细胞2(Th2)因子中的白细胞介素4(IL-4)以及TRAF6、NGFR水平;采用Pearson法分析血清TRAF6、NGFR表达以及二者与甲状腺功能指标、炎症因子的相关性。结果HT正常组、HT减退组和HT亢进组TRAF6的表达水平显著高于对照组[(25.12±3.36)µg/L、(28.16±3.48)µg/L、(22.65±3.27)µg/L比(19.25±2.42)µg/L],HT正常组和HT减退组表达水平显著高于HT亢进组,HT减退组显著高于HT正常组(P<0.05);HT正常组、HT减退组和HT亢进组NGFR的表达水平显著低于对照组[(27.36±3.48)µg/L、(25.02±3.42)µg/L、(29.74±3.56)µg/L比(32.04±3.71)µg/L],HT正常组和HT减退组表达水平显著低于HT亢进组,HT减退组显著低于HT正常组(P<0.05);Pearson相关性分析结果显示,HT病人血清中TRAF6和NGFR表达水平呈负相关(P<0.05);与对照组相比,HT组血清中IL-2、IFN-γ、TPOAb、TGAb、游离三碘甲状原氨酸、血清三碘甲状原氨酸、甲状腺素、甲状腺球蛋白水平显著升高,IL-4、游离甲状腺素水平显著降低(P<0.05);Pearson法分析显示,TRAF6水平与IL-2、IFN-γ、TPOAb、TGAb、游离三碘甲状原氨酸、血清三碘甲状原氨酸、甲状腺素、甲状腺球蛋白水平呈正相关,与IL-4、游离甲状腺素水平呈负相关(P<0.05),NGFR水平与IL-4、游离甲状腺素水平呈正相关,与IL-2、IFN-γ、TPOAb、TGAb、游离三碘甲状原氨酸、血清三碘甲状原氨酸、甲状腺素、甲状Objective To investigate the correlation between serum tumor necrosis factor receptor-related factor 6(TRAF6)and nerve growth factor receptor(NGFR)levels and immune levels and thyroid function in children with Hashimoto´s thyroiditis(HT).Methods The prospective study included 104 children with HT who were admitted to Chengde Maternal and Child Health Hospital from March 2021 to March 2022 as the HT group.According to the thyroid function,they were divided into 30 cases in HT hyperactive group,38 cases in HT normal group and 36 cases in HT decompensated group.Ninety-five healthy children who were physically examined in the pediatric outpatient clinic were randomly selected as the control group.The levels of interleukin-2(IL-2)andγ-interferon(IFN-γ)in serum helper T-cell 1(Th1)factor,interleukin 4(IL-4)in helper T-cell 2(Th2)factor,and TRAF6 and NGFR were measured by enzyme-linked immunosorbent assay(ELISA);Pearson method was used to analyze serum TRAF6 and NGFR expression and the correlation between the two and thyroid function indexes and inflammatory factors.Results The expression level of TRAF6 in normal HT group,hypoactive HT group and hyperactive HT group was significantly higher than that in control group[(25.12±3.36)μg/L,(28.16±3.48)μg/L,(22.65±3.27)μg/L vs.(19.25±2.42)μg/L],the expression level in normal HT group and hypoactive HT group was significantly higher than that in hyperactive HT group,and the expression level in hypoactive HT group was significantly higher than that in normal HT group(P<0.05).The expression level of NGFR in normal HT group,hypoactive HT group and hyperactive HT group was significantly lower than that in control group[(27.36±3.48)μg/L,(25.02±3.42)μg/L,(29.74±3.56)μg/L vs.(32.04±3.71)μg/L],the expression level in normal HT group and hypoactive HT group was significantly lower than that in hyperactive HT group,and the expression level in hypoactive HT group was significantly lower than that in normal HT group(P<0.05).Pearson correlation analysis showed that there

关 键 词：慢性淋巴细胞性甲状腺炎 肿瘤坏死因子受体相关因子6 神经生长因子受体 免疫水平 甲状腺功能 

分 类 号：R725.8[医药卫生—儿科]