干旱胁迫下苎麻生理指标和抗逆相关基因表达的变化  

Changes of physiological indexes and expression of stress resistance related genes in ramie under drought stress

作　　者：张奥深 徐敏 陈炼 熊伟 程长松 汪红武 ZHANG Ao-shen;XU Min;CHEN Lian;XIONG Wei;CHENG Chang-song;WANG Hong-wu(Xianning Academy of Agricultural Sciences/Hubei Ramie Engineering Technology Center,Xianning 437100,Hubei,China;Xianning Xiangcheng Characteristic Agricultural Technology Research Institute Co.,Ltd.,Xianning 437100,Hubei,China)

机构地区：[1]咸宁市农业科学院/湖北省苎麻工程技术中心,湖北咸宁437100 [2]咸宁香城特色农业技术研究院有限公司,湖北咸宁437100

出　　处：《湖北农业科学》2024年第5期6-11,共6页Hubei Agricultural Sciences

基　　金：国家现代农业产业技术体系资助项目(CARS-16-S10)。

摘　　要：为研究干旱胁迫对苎麻(Boehmeria nivea L.)生理特性和抗逆相关基因的影响,以苎麻品种华苎4号为试验材料,采用自然干旱胁迫,测定其生理指标、抗逆相关基因表达量和相关农艺性状的变化。结果表明,与对照相比,干旱胁迫处理苎麻叶片叶绿素SPAD降低,茎尖和叶片的相对电导率、游离脯氨酸含量、丙二醛含量和可溶性糖含量均显著增加(P<0.05),超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)的活性均升高,抗逆相关基因BnP5CS1、BnWRKY1、BnACO1、Bn-α-amylase、BnAPX1、BnbZIP1、BnDREB19和BnGR1的相对表达量均提高,其中BnP5CS1和BnWRKY1在叶片中的相对表达量分别提高了5.1倍和5.3倍;与对照相比,干旱胁迫处理苎麻株高、茎粗、皮厚、地上部鲜重和单株原麻重均显著下降(P<0.05),茎粗和皮厚分别降低0.13 cm和0.14 mm,地上部鲜重和单株原麻重分别降低了52.05%和39.53%。To study the effects of drought stress on physiological characteristics and stress resistance related genes of ramie(Boehme-ria nivea L.),the physiological indexes,stress resistance related gene expression and agronomic traits of Huazhu No.4 were measured by natural drought stress.The results showed that,compared with the control,the chlorophyll SPAD of ramie leaves decreased under drought stress,the relative conductivity,free proline content,malondialdehyde content and soluble sugar content of stem tip and leaves increased significantly(P<0.05),the activities of superoxide dismutase(SOD),catalase(CAT)and peroxidase(POD)in-creased,and the relative expression levels of stress-related genes BnP5CS1,BnWRKY1,BnACO1,Bn-α-amylase,BnAPX1,Bn-bZIP1,BnDREB19 and BnGR1 were increased.The relative expressions of BnP5CS1 and BnWRKY1 in leaves were increased 5.1 and 5.3 times compared with control.Compared with the control,the plant height,stem diameter,skin thickness,fresh aboveground weight and raw hemp weight per plant under drought stress were significantly decreased(P<0.05),the stem diameter and skin thick-ness were decreased by 0.13 cm and 0.14 mm,respectively,and the fresh aboveground weight and raw hemp weight per plant were de-creased by 52.05%and 39.53%,respectively.

关 键 词：苎麻(Boehmeria nivea L.) 干旱胁迫 生理指标 抗逆基因 相对表达量 

分 类 号：S563.1[农业科学—作物学]