基于SSR荧光标记的121份野生枸杞种质遗传结构解析  

Genetic structure analysis of 121 wild goji berry germplasms based on SSR fluorescence markers

作　　者：戴国礼 刘娜 秦垦 张波 尹跃 米佳 何昕孺 DAI Guo-i;LIU Na;QIN Ken;ZHANG Bo;YIN Yue;MI Ja;HE Xin-ru(Institute of Goji Berry Science,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 740002,China)

机构地区：[1]宁夏农林科学院枸杞科学研究所,银川740002

出　　处：《湖北农业科学》2024年第5期207-214,共8页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(32060360);宁夏重点研发计划项目(2020BBF02006;2021BEF02005)。

摘　　要：以采集的121份野生枸杞(Lycium barbarum L.)种质为材料,用9对SSR引物进行扩增,采用GeneALEX、Power Marker软件进行多态性分析,利用NTSYS、STRUC-TURE软件进行遗传多样性分析、聚类分析和主成分分析。结果表明,9对SSR引物共检测到108个等位基因,等位基因数(Na)、有效等位基因数(Ne)、观测杂合度(Ho)、期望杂合度(He)、多态信息含量(PIC)、Shannon’s信息指数分别为12个、4.620个、0.678、0.651、0.629、1.568;聚类结果显示121份野生枸杞种质可划分为5个亚群,群体遗传结构表明当K=5时,121个种质划分为5个亚群,来自宁夏的NXGY-02、NXGY-032个种质与来自内蒙古的6个种质之间的遗传距离最远,亲缘关系较远,来自青海省、新疆、甘肃省及内蒙古的其余野生枸杞种质之间存在广泛的基因交流现象;宁夏、内蒙古的野生枸杞种质具有独立起源进化的可能,而新疆、甘肃省、青海省的种质与宁夏种质亲缘关系较近,很可能属于同一起源。121 wild goji berry(Lycium barbarum L.)germplasms were collected as materials,9 pairs of SSR primers were used of am-plification,and polymorphism was analyzed using GeneALEX and Power Marker software.Genetic diversity analysis,cluster analy-sis,and principal component analysis were performed using NTSYS and STRUC-TURE software.The results showed that a total of 108 alleles were detected using 9 pairs of SSR primers.The number of alleles(Na),effective alleles(Ne),observed heterozygosity(Ho),expected heterozygosity(He),polymorphic information content(PIC),and Shannon’s information index were 12,4.620,0.678,0.651,0.629,and 1.568,respectively;the clustering results showed that 121 wild goji berry germplasms could be divided into 5 sub-groups.The genetic structure of the population showed that when K=5,121 germplasms could be divided into 5 subgroups.The genetic distance between NXGY-02 and NXGY-03 germplasms from Ningxia and 6 germplasms from Inner Mongolia was the farthest,and the genetic relationship was relatively distant,there was extensive gene exchange among other wild goji berry germplasms from Qinghai Province,Xinjiang,Gansu Province,and Inner Mongolia;the wild goji berry germplasms in Ningxia and Inner Mongolia had the possi-bility of independent origin and evolution,while the germplasms in Xinjiang,Gansu,and Qinghai provinces were closely related to the germplasms in Ningxia and were likely to belong to the same origin.

关 键 词：野生枸杞(Lycium barbarum L.) 种质 SSR荧光标记 遗传结构 

分 类 号：S326[农业科学—作物遗传育种]