美味猕猴桃MYB家族鉴定及其对水淹胁迫的响应  

Identification of the MYB family of Actinidia deliciosa and its response to flooding stress

作　　者：乐有章 王建军[2] 戢小梅 迟文超 林之希 YUE You-zhang;WANG Jian-jun;JI Xiao-mei;CHI Wen-chao;LIN Zhi-xi(Wuhan Academy of Agricultural Sciences,Wuhan 430075,China;College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]武汉市农业科学院,武汉430075 [2]南京农业大学园艺学院,南京210095

出　　处：《湖北农业科学》2024年第5期215-222,共8页Hubei Agricultural Sciences

摘　　要：基于美味猕猴桃(Actinidia deliciosa)二代转录组测序数据,通过多种生物信息学手段鉴定和分析了AcMYB家族基因。结果表明,在美味猕猴桃转录组数据中共获得64个具有完整ORF序列的AcMYB家族成员,将其分成4个亚族,其中亚族Ⅰ共34个成员,亚族Ⅱ共18个成员,亚族Ⅲ共10个成员,亚族Ⅳ共2个成员。水淹处理前后表达分析显示,AcMYB_214187、AcMYB_25788等AcMYB成员美味猕猴桃根中的表达水平明显降低,快速响应水淹胁迫,而叶中的表达水平差异不明显。AcMYB_19450、AcMYB_30021等AcMYB成员根中的表达水平差异不明显,但叶中的表达水平明显降低,可能原因是根接收到低氧胁迫信号后,将其传递到地上部并发挥作用,通过转录水平调控美味猕猴桃对低氧胁迫的响应。研究结果为后续开展美味猕猴桃MYB转录因子研究、促进抗逆境育种、提高产量提供了依据。Based on the second-generation transcriptome sequencing data of Actinidia deliciosa,AcMYB family genes were identified and analyzed using various bioinformatics methods.The results showed that a total of 64 AcMYB family members with complete ORF sequences were obtained from the transcriptome data of Actinidia deliciosa,which were divided into 4 subgroups.Among them,sub-group I had 34 members,subgroup II had 18 members,subgroup III had 10 members,and subgroup IV had 2 members.Expression analysis before and after waterlogging treatment showed that the expression levels of AcMYB members such as AcMYB214187 and Ac-MYB25788 were significantly reduced in the roots of Actinidia deliciosa,responding quickly to waterlogging stress,while there was no significant difference in expression levels in the leaves.The expression levels in the roots of AcMYB members such as AcMYB19450 and AcMYB30021 were not significantly different,but the expression levels in the leaves were significantly reduced.This might be due to the fact that the roots,upon receiving hypoxic stress signals,transmitted them to the aboveground parts and exerted their ef-fects,regulating the response of Actinidia deliciosa to hypoxic stress at the transcriptional level.The research results provided a basis for further research on MYB transcription factors in Actinidia deliciosa,promoting stress resistance breeding,and improving yield.

关 键 词：美味猕猴桃(Actinidia deliciosa) AcMYB 鉴定 水淹胁迫 响应 

分 类 号：S663.4[农业科学—果树学]