卡巴胆碱对失血性休克大鼠肺微血管内皮细胞功能的影响  

Effect of Carbachol on the Function of Pulmonary Microvascular Endothelial Cells in Rats with Hemorrhagic Shock

作　　者：陈超[1] 王化冰 索小燕[1] 李征[1] 张辉[1] 李方方 CHEN Chao;WANG Huabing;SUO Xiaoyan;LI Zheng;ZHANG Hui;LI Fangfang(Department of Anesthesiology,Henan Provincial People’s Hospital,Zhengzhou 450003,China;Department of Spinal and Spinal Cord Surgery,Henan Provincial People’s Hospital,Zhengzhou 450003,China)

机构地区：[1]河南省人民医院麻醉与围术期医学科,河南郑州450003 [2]河南省人民医院脊柱脊髓外科一,河南郑州450003

出　　处：《河南医学研究》2024年第11期1935-1939,共5页Henan Medical Research

摘　　要：目的 观察大鼠失血性休克时卡巴胆碱对肺微血管内皮细胞的作用。方法 将30只健康雄性SD大鼠(SPF级)按每组10只随机分为假休克组(SS组)、失血性休克组(HS组)、失血性休克+卡巴胆碱组(CBL组)。失血性休克动物模型按照Wiggers改良法制作,各组模型制备完毕后6 h,经右侧股动脉采血,检测肿瘤坏死因子-α(TNF-α)水平,取右肺下叶肺组织检测Src抑制的蛋白激酶C的底物(SSeCKS)、E-选择素蛋白质水平,取右肺下叶行透射电镜检查肺微血管内皮细胞超微结构。结果 SS组表达少量的TNF-α、SSeCKS、E-选择素,HS组和CBL组表达升高(P<0.05),与HS组相比,CBL组表达降低(P<0.05)。SS组肺微血管内皮细胞细胞膜表面光滑,细胞膜、细胞质、细胞核形态结构正常;HS组肺微血管内皮细胞出现细胞水肿、坏死和凋亡表现(细胞膜表面皱折、细胞膜表面凹凸不平、细胞器水肿、异染色质染色加深边集等);CBL组较SS组,细胞有所损伤,但好于HS组。结论 大鼠失血性休克时卡巴胆碱对肺微血管内皮细胞具有保护作用,也可通过抑制炎症细胞合成和释放炎症介质减轻对血管内皮细胞的损伤。Objective To observe the effect of carbachol on pulmonary microvascular endothelial cells in rats with hemorrhagic shock.Methods Thirty healthy male SD(SPF grade)rats were randomly divided into three groups as follows at 10 rats per group:sham shock group(SS group),haemorrhagic shock group(HS group),haemorrhagic shock group+carbachol group(CBL group).The animal models of haemorrhagic shock were made according to the Wiggers modified method.Six hours after the preparation of the models in each group,blood was collected via the right femoral artery to detect the level of tumour necrosis factor-α(TNF-α),and the lung tissues of the lower lobe of the right lung were taken to detect the levels of Src-suppressed protein kinase C substrate(SSeCKS)and E-selectin proteins,and the lower lobe of the right lung was taken to be examined by transmission electron microscopy.Results The SS group expressed a small amount of TNF-α,SSeCKS and E-selectin,and their expression was elevated in the HS and CBL groups(P<0.05),and their expression was reduced in the CBL group compared with the HS group(P<0.05).The cell membrane surface of lung microvascular endothelial cells in the SS group was smooth,and the morphology and structure of cell membrane,cytoplasm,and nucleus were normal.The lung microvascular endothelial cells in the HS group showed cellular edema,necrosis,and apoptosis manifestations(folding of the cell membrane surface,unevenness of the cell membrane surface,edema of organelles,and deepening of heterochromatin staining in the border set,etc.).In the CBL group,compared with the SS group,there were cellular damages but they were significantly better than that in the HS group.Conclusion Carbachol can exert a protective effect on pulmonary microvascular endothelial cells of rats in hemorrhagic shock.It may also attenuate damage to vascular endothelial cells by inhibiting inflammatory cell synthesis and release of inflammatory mediators.

关 键 词：失血性休克 肺微血管内皮细胞 卡巴胆碱 SSECKS E-选择素 

分 类 号：R3[医药卫生—基础医学]