含羞草根乙酸乙酯部位对急性髓系白血病小鼠的影响  

Effect of ethyl acetate extract from Mimosa pudica root on acute myeloid leukemia in mice

作　　者：邓婉柔 李慧敏 曾元宁 王秋红 DENG Wanrou;LI Huimin;ZENG Yuanning;WANG Qiuhong(School of Traditional Chinese Medicine/Guangdong Engineering Technology Research Center for Standardized Processing of Chinese Herbal Pieces,Guangdong Pharmaceutical University,Guangzhou 510006,China;Key Laboratory of Northern Medicine Foundation and Application under Ministry of Education/Heilongjiang Key Laboratory of Traditional Chinese Medicine and Natural Medicine Pharmacodynamic Material Base,Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]广东药科大学中药学院/广东省中药饮片规范化炮制工程技术研究中心,广州510006 [2]黑龙江中医药大学教育部北药基础与应用研究重点实验室/黑龙江省中药及天然药物药效物质基础研究重点实验室,哈尔滨150040

出　　处：《中国药房》2024年第11期1315-1319,共5页China Pharmacy

基　　金：国家重点研发计划项目(No.2018YFC1707100);广东省重点领域研发计划项目(No.2020B111112-0002)。

摘　　要：目的 研究含羞草根乙酸乙酯部位(简称为乙酸乙酯部位)对急性髓系白血病小鼠的影响。方法 以不同质量浓度(0.062 5、0.125、0.25、0.5 mg/mL)乙酸乙酯部位作用于急性粒-单核细胞白血病细胞WEHI-3,考察其对该细胞活力的影响。将50只BALB/C小鼠随机分为空白对照组、模型组、阳性对照组(5-氟尿嘧啶,13 mg/kg)和乙酸乙酯部位低、高剂量组(50、200 mg/kg),每组10只。除空白对照组外,其余各组小鼠均腹腔注射WEHI-3细胞悬液进行造模,并在造模第2天起灌胃相应药物/水,每天1次,连续14 d。末次给药后,检测小鼠肝、脾指数,并进行肝组织病理学形态观察和血液学分析以及白细胞分化检测;检测小鼠血清中细胞因子[白细胞介素2(IL-2)、IL-3、干扰素γ(IFN-γ)、肿瘤坏死因子α(TNF-α)]水平;检测小鼠全血中白细胞表面标志物[分化簇3(CD3)、CD19、CD11b、分化簇107b(即Mac-3)]抗体水平。结果 经0.062 5~0.5 mg/mL的乙酸乙酯部位作用后,WEHI-3细胞增殖抑制率均显著升高(P<0.05)。经高剂量的乙酸乙酯部位干预后,小鼠的肝、脾指数,血清中TNF-α水平,全血中白细胞表面CD11b、Mac-3抗体水平均显著降低(P<0.05),血清中IL-2、IL-3、IFN-γ水平和全血中白细胞表面CD3、CD19抗体水平均显著升高(P<0.05);肝实质偶有淋巴细胞浸润,几乎无炎症细胞浸润;血液学指标改善且白细胞分化减弱。结论 含羞草根乙酸乙酯部位可抑制WEHI-3细胞增殖,改善急性髓系白血病小鼠症状,其作用机制与增强机体免疫功能有关。OBJECTIVE To study the inhibitory effect of ethyl acetate extract from Mimosa pudica root(ethyl acetate extract for short)on acute myeloid leukemia in mice.METHODS Different concentrations of ethyl acetate extract(0.0625,0.125,0.25,0.5 mg/mL)were used to treat acute myelomonocytic leukemia cell lines WEHI-3,and their effects on cell viability were investigated.Fifty BALB/C mice were randomly divided into blank control group,model group,positive control group(5-fluorouracil,13 mg/kg),and ethyl acetate extract low-dose and high-dose groups(50,200 mg/kg),with 10 mice in each group.Except for the blank control group,the leukemia model was constructed by intraperitoneal injection of WEHI-3 cells in other groups,and from the second day of modeling,corresponding drugs/water were orally administered once a day for 14 consecutive days.After the last administration,the liver and spleen indexes of mice were measured,and liver tissue pathological morphology observation,hematological analysis,and white blood cell differentiation detection were performed;the levels of cytokine[interleukin-2(IL-2),IL-3,interferon-γ(IFN-γ),tumor necrosis factor-α(TNF-α)]in serum were determined;the levels of leukocyte surface markers[cluster of differentiation 3(CD3),CD19,CD11b,CD107b(Mac-3)]in whole blood were all detected.RESULTS After treated with 0.0625-0.5 mg/mL ethyl acetate,the inhibition rate of cell proliferation were increased significantly(P<0.05).After intervention with high-dose ethyl acetate,the liver and spleen index,serum level of TNF-α,the levels of CD11b and Mac-3 in blood were significantly reduced(P<0.05),while serum levels of IL-2,IL-3 and and the levels of CD3 and CD19 in blood were increased significantly(P<0.05).Occasional lymphocyte infiltration was present in the liver parenchyma,with almost no infiltration of inflammatory cells;hematology improvement and weakened white blood cell differentiation were found.CONCLUSIONS The ethyl acetate extract of M.pudica root can inhibit the proliferation of WEHI-3 cells,and impr

关 键 词：含羞草根 乙酸乙酯部位 急性髓系白血病 白细胞分化 免疫功能 

分 类 号：R965[医药卫生—药理学] R285[医药卫生—药学]