宏基因组二代测序诊断免疫缺陷患者感染性疾病的研究进展  

Research progress of infectious diseases in immunocompromised hosts using metagenomic next generation sequencing-based diagnostics

作　　者：杨震宇 於江泉 YANG Zhenyu;YU Jiangquan(Yangzhou University,Yangzhou,Jiangsu,225009;Department of Emergency Medicine,the Seventh Clinical Medical College of Yangzhou University,Jingjiang People′s Hospital,Jingjiang,Jiangsu,214500;School of Clinical Medicine of Yangzhou University,Department of Critical Care Medicine,Northern Jiangsu People′s Hospital Affiliated to Yangzhou University,Yangzhou,Jiangsu,225000)

机构地区：[1]扬州大学,江苏扬州225009 [2]扬州大学第七临床医学院/靖江市人民医院急诊科,江苏靖江214500 [3]扬州大学临床医学院/扬州大学附属苏北人民医院重症医学科,江苏扬州225000

出　　处：《实用临床医药杂志》2024年第9期129-133,共5页Journal of Clinical Medicine in Practice

基　　金：江苏省十四五医学重点学科建设单位基金资助项目(JSDW202217);江苏省扬州市社会发展项目(YZ2023105)。

摘　　要：宏基因组二代测序(mNGS)通过识别临床样本中的微生物核酸来协助诊断,是用于免疫功能低下患者感染性疾病诊断的有效工具。mNGS能够在常规检测为阴性的患者中识别出致病生物体,但目前在免疫力低下患者中,评估mNGS诊断感染效能的报告仅限于个体患者或小型回顾性研究。本文回顾分析文献,为在临床中开展mNGS相关研究提供新思路。Metagenomic next generation sequencing(mNGS)based diagnostics that identify microbial nucleic acids in clinical samples may be a useful tool in addressing some of these challenges.Studies of mNGS in immunocompromised hosts have demonstrated that these diagnostics are capable of identifying causative organisms in a subset of patients for whom conventional testing has been negative.But the reports evaluating the diagnostic efficiency of mNGS in immunocompromised patients are limited to individual patients or small retrospective studies at present.This article reviewed and analyzed the literature to provide new ideas for conducting related research on mNGS in clinical practice.

关 键 词：免疫功能不全 感染性疾病 病原体 宏基因组二代测序 液体活检 感染监测 

分 类 号：R633[医药卫生—外科学] R363[医药卫生—临床医学] R446