基于“去腐生肌”法探讨升白灵药纱条促进肛周脓肿术后创面愈合的动物实验研究  

An Animal Experimental Study Based on the Eliminating Necrotic Tissues and Promoting Granulation Method Explores the Promotion of Wound Healing in the Perianal Abscess Surgery by Shengbailing Medication Yarn Strip

作　　者：廖武[1] 葛巍[1] 陈光华[1] 张磊昌[1] 左铮云[2] LIAO Wu;GE Wei;CHEN Guanghua;ZHANG Leichang;ZUO Zhengyun(Affiliated Hospital of Jiangxi University of Chinese Medicine,Nanchang Jiangxi 330006,China;Formula-pattern Research Center,Jiangxi University of Chinese Medicine,Nanchang Jiangxi 330004,China)

机构地区：[1]江西中医药大学附属医院,江西南昌330006 [2]江西中医药大学方-证研究中心,江西南昌330004

出　　处：《药品评价》2024年第2期147-152,共6页Drug Evaluation

基　　金：江西中医药管理局科技计划(2022B918);2022年度江西省名老中医药专家学术经验继承项目。

摘　　要：目的 观察升白灵药对肛周脓肿术后创面愈合的影响。方法 将40只SD大鼠分为空白组、模型组、雷夫诺尔组和升白灵药组,除空白组外其他三组用粪菌液造肛周脓肿模型,给药至大鼠创面完全愈合。观察大鼠创面愈合时间,第5天、10天、15天的闭合指数,创面第3天和第15天的白细胞水平,创面VEGF、b-FGF表达水平和炎症因子TNF-α、IL-6的表达水平。结果 升白灵药组和雷夫诺尔组大鼠创面完全愈合时间与模型组相比显著缩短(P<0.01),且升白灵组大鼠创面愈合时间短于雷夫诺尔组(P<0.01);升白灵药组和雷夫诺尔组大鼠创面第5天、10天、15天的闭合指数大于模型组(P<0.01),升白灵药组大鼠创面第5天、10天、15天的闭合指数均大于雷夫诺尔组(P<0.01);与空白组相比,3个造模组第3天白细胞计数均上升(P<0.01),与其余两组相比,升白灵药组白细胞上升更加显著(P<0.01);第15天雷夫诺尔组和升白灵药组大鼠白细胞计数低于模型组(P<0.01),与雷夫诺尔组相比,升白灵药组白细胞下降更加明显(P<0.01);升白灵药组大鼠背部创面组织VEGF的表达水平高于模型组和雷夫诺尔组(P<0.01);升白灵药组、雷夫诺尔组大鼠背部创面组织b-FGF的表达水平较模型组升高(P<0.01);升白灵药组b-FGF的表达水平较雷夫诺尔组升高(P<0.01);升白灵药组、雷夫诺尔组促炎因子IL-6、TNF-α的表达水平较模型组明显降低(P<0.01);升白灵药组大鼠背部创面组织促炎因子IL-6、TNF-α的表达水平较之雷夫诺尔组降低(P<0.01)。结论 以“去腐生肌”为遣方原则的升白灵药纱条能够通过调节炎症因子水平,上调VEGF、b-FGF表达促进肛周脓肿创面愈合。Objective This study aimed to observe the effect of shengbailing medication on wound healing in perianal abscess surgery.Methods 40 SD rats divided into four groups,including a blank group,a model group,a rayonel group,and a shengbailing medication group.Except for the blank group,the other three groups received fecal bacteria injection to create a perianal abscess model and were administered medication until the rats'wounds completely healed.Observed the time of wound healing,closure index on the 5th,10th,and 15th days,white blood cell levels on the 3rd and 15th days,and the expression levels of VEGF,b-FGF,and the inflammatory factors TNF-αand IL-6 at the wound site.Results The time for complete wound healing in the shengbailing medication group and rayonel group rats was significantly shorter compared to the model group(P<0.01),and the shengbailing group had a shorter healing time than the rayonel group(P<0.01).The closure index on the 5th,10th,and 15th days at the wound site of rats in the shengbailing medication group and rayonel group was significantly higher than the model group(P<0.01),and the closure index in the shengbailing medication group was higher than the rayonel group on all three days(P<0.01).Compared to the a blank group,all three modeling groups showed a significant increase in white blood cell count on the 3rd day(P<0.01),and the white blood cell increase in the shengbailing medication group was more significant(P<0.01).On the 15th day,the white blood cell count of rats in the rayonel group and shengbailing medication group was lower than that of the model group(P<0.01),and the decrease in white blood cells was more significant in the shengbailing medication group than the rayonel group(P<0.01).The expression level of VEGF in the wound tissue of rats in the shengbailing medication group was significantly higher than that in the model group and rayonel group(P<0.01).The expression level of b-FGF in the wound tissue of rats in the shengbailing medication group and rayonel group was significantl

关 键 词：肛周脓肿 升白灵药 去腐生肌 炎症因子 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学]