不同分期痛风性关节炎患者NALP3炎性体及外周血γδT细胞水平观察  

Observation of NALP3 inflammasome and peripheral bloodγδT cell levels in gouty arthritis patients with different stages

作　　者：王晓磊[1] 武晔[1] 刘丹[1] 郭圆圆 王金 侯艳辉 胡丽伟[1] 苏杏丽 WANG Xiao-lei;WU Ye;LIU Dan(Department of Rheumatology,Hebei Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine,Cangzhou Hebei 061000,China)

机构地区：[1]河北省沧州中西医结合医院风湿科,河北沧州061000

出　　处：《临床和实验医学杂志》2024年第9期930-934,共5页Journal of Clinical and Experimental Medicine

基　　金：河北省中医药类科学研究课题计划项目(编号:2024465)。

摘　　要：目的观察不同分期痛风性关节炎(GA)患者NALP3炎性体及外周血γδT细胞水平。方法回顾性选取2022年7月至2023年7月在河北省沧州中西医结合医院接受治疗的96例GA患者作为研究对象。依据GA临床分期,分为急性关节炎期组(n=38)、间歇期组(n=26)、慢性关节炎期组(n=32)。另选取同期于本院体检中心体检的健康者35名设为对照组。观察各组NALP3炎性体及外周血γδT细胞水平,分析各组GA患者的临床指标,包括血尿酸、血肌酐、总胆固醇、甘油三酯、血清C反应蛋白(CRP)水平及白细胞计数(WBC)。采用Pearson相关性分析急性关节炎期患者外周血γδT细胞及其亚型、NALP3炎性体与临床指标的关系。结果各组NALP3、γδT1细胞比较,差异均无统计学意义(P>0.05);各组Caspase-1、ASC、γδT细胞、γδT2细胞比较,差异均有统计学意义(P<0.05),其中,急性关节炎期组、间歇期组、慢性关节炎期组Caspase-1水平均高于对照组,γδT细胞和γδT2细胞比例均低于对照组,且病情程度越高,Caspase-1水平越高,γδT细胞和γδT2细胞比例越低;急性关节炎期组、间歇期组、慢性关节炎期组ASC水平均高于对照组,但急性关节炎期组低于间歇期组、慢性关节炎期组,差异均有统计学意义(P<0.05)。各组总胆固醇、甘油三酯、CRP水平比较,差异均无统计学意义(P>0.05);各组血尿酸、WBC、血肌酐水平比较,差异均有统计学意义(P<0.05),其中,急性关节炎期组、间歇期组、慢性关节炎期组的血尿酸、WBC、血肌酐水平均高于对照组,差异均有统计学意义(P<0.05),且病情程度越高,血尿酸、WBC、血肌酐水平越高。相关性分析显示,γδT细胞在淋巴细胞中的比例及γδT1细胞在γδT细胞中的比例与血尿酸、血肌酐、总胆固醇、甘油三酯、WBC、CRP指标无明显相关性;γδT2在γδT细胞中的比例与甘油三酯、WBC呈负相关(P<0.05)。相关性分析显示,NALP3 mRNA表达�Objective To observe the levels of NALP3 inflammasome and peripheral bloodγδT cells in gouty arthritis(GA)patients with different stages.Methods A total of 96 GA patients treated in Hebei Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine from July 2022 to July 2023 were selected and studied.According to the clinical stages of GA,they were divided into the acute arthritis stage group(n=38),the intermittent arthritis stage group(n=26)and the chronic arthritis stage group(n=32).Another 35 healthy individuals who underwent physical examinations at the same time at the medical examination center of our hospital were selected as the control group.The levels of NALP3 inflammasome and peripheral bloodγδT cells in each group were observed.The clinical indicators of GA patients in each group were analyzed,including the levels of serum uric acid,serum creatinine,total cholesterol,triglyceride,serum C-reactive protein(CRP)and white blood cell count(WBC).Pearson correlation was used to analyze the relationship between peripheral blood gamma-delta T cells and their subtypes,NALP3 inflammasome and clinical indexes in patients with acute arthritis.Results There was no statistically significant difference in the comparison of NALP3 andγδT1 cells in each group(P>0.05);there were statistically significant differences in Caspase-1,ASC,γδTcells,andγδT2 cells among the groups(P<0.05).Among them,the levels of Caspase-1 in the acute arthritis stage group,intermittent stage group,and chronic arthritis stage group were higher than those in the control group,and the proportion ofγδT cells andγδT2 cells was lower than that in the control group,the higher the severity of the disease,the higher the Caspase-1 level,and the lower the proportion ofγδT cells andγδT2 cells;the ASC levels in the acute arthritis stage group,intermittent stage group,and chronic arthritis stage group were higher than those in the control group,but the acute arthritis stage group was lower than those in the intermittent stage gr

关 键 词：痛风性关节炎 NALP3炎性体 外周血ΓΔT细胞 急性关节炎期 间歇期 慢性关节炎期 

分 类 号：R589.7[医药卫生—内分泌]