红果樫木叶木脂素类化学成分研究  

Lignan constituents from the leaves of Dysoxylum gotadhora

作　　者：闫慧娇 陈雨洁 宋祥云 李恩霞 耿岩玲 YAN Huijiao;CHEN Yujie;SONG Xiangyun;LI Enxia;GENG Yanling(Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province,Shandong Analysis and Test Center,School of Pharmaceutical Sciences,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250014,China;Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province,School of Pharmaceutical Sciences,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250014,China;Shandong Center for Food and Drug Evaluation and Inspection,Jinan 250014,China)

机构地区：[1]齐鲁工业大学(山东省科学院)山东省分析测试中心山东省大型精密分析仪器应用技术重点实验室,山东济南250014 [2]齐鲁工业大学(山东省科学院)药学院山东省高等学校天然药物活性成分研究重点实验室,山东济南250014 [3]山东省食品药品审评查验中心,山东济南250014

出　　处：《山东科学》2024年第3期10-17,共8页Shandong Science

基　　金：国家自然科学基金(32200311);济南市高校20条(202228020);齐鲁工业大学(山东省科学院)科教产融合创新试点工程项目(2022PX029)。

摘　　要：研究红果樫木叶的化学成分,运用大孔树脂、硅胶、Sephadex LH-20和半制备色谱等多种色谱技术从红果樫木叶70%乙醇提取物中分离得到4个木脂素类化合物。运用1 H NMR(核磁共振)、13 C NMR和MS(质谱)波谱学数据等分别鉴定为4,4′-dihydroxy-3,3′,5,5′-trtramethoxy-7,9′:7′,9-diepoxylignane(1),4′-hydroxy-3,4,5,3′-trtramethoxy-7,9′:7′,9-diepoxylignane(2),4′,4″-dihydroxy-3,3′,3″,5,5′,5″-hexamethoxy-7,9′:7′,9-diepoxy-4,8″-oxy-8,8′-sesquineolignan-7″,9″-diol(3)和4′,4″-dihydroxy-3,3′,3″,5,5′-pentamethoxy-7,9′:7′,9-diepoxy-4,8″-oxy-8,8′-sesquineolignan-7″,9″-diol(4)。化合物1~4均首次从樫木属植物中分离得到。使用DPPH(1,1-二苯基-2-三硝基苯肼)自由基清除率测定化合物的抗氧化活性,结果显示木脂素类化合物1~4具有良好的DPPH自由基清除能力。To study the constituents of the leaves of Dysoxylum gotadhora,the 70%ethanol extract was separated using macroporous resin column chromatography,silica gel column chromatography,Sephadex LH-20 gel column chromatography,combined with semi-preparative HPLC and other methods.Four compounds 4,4′-dihydroxy-3,3′,5,5′-trtramethoxy-7,9′:7′,9-diepoxylignane(1),4′-hydroxy-3,4,5,3′-trtramethoxy-7,9′:7′,9-diepoxylignane(2),4′,4″-dihydroxy-3,3′,3″,5,5′,5″-hexamethoxy-7,9′:7′,9-diepoxy-4,8″-oxy-8,8′-sesquineolignan-7″,9″-diol(3),4′,4″-dihydroxy-3,3′,3″,5,5′-pentamethoxy-7,9′:7′,9-diepoxy-4,8″-oxy-8,8′-sesquineo lignan-7″,9″-diol(4)were isolated.Their structures were established by 1 H NMR,13 C NMR and MS spectroscopy techniques.Compounds 1 to 4 were obtained from the genus Dysoxylum for the first time.DPPH(1,1-diphenyl-2-picryhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl)radical scavenging rate were used to determine the antioxidant activity.The results show that lignans have significant DPPH free radical scavenging ability.

关 键 词：红果樫木 化学成分 结构鉴定 

分 类 号：R284.1[医药卫生—中药学]