Nb_(2)C MXene薄膜眼部植入体的生物相容性  

Biocompatibility of Nb_(2)C MXene film for ophthalmic implants

作　　者：马巍 彭立 陈百华[1,2] Wei MA;Li PENG;Bai-hua CHEN(Department of Ophthalmology,The Second Xiangya Hospital of Central South University,Changsha 410011,China;Hunan Clinical Research Center of Ophthalmic Disease,Changsha 410011,China;Department of Ophthalmology,Haikou Affiliated Hospital of Central South University Xiangya School of Medicine,Haikou 570311,China)

机构地区：[1]中南大学湘雅二医院眼科,长沙410011 [2]湖南省眼科临床研究中心,长沙410011 [3]中南大学湘雅医学院附属海口医院眼科,海口570311

出　　处：《Transactions of Nonferrous Metals Society of China》2024年第5期1618-1633,共16页中国有色金属学报（英文版）

基　　金：National Natural Science Foundation of China(Nos.82171030,81870678);Natural Science Foundation of Hunan Province,China(No.2021JJ30925);Health Commission of Hunan Province,China(No.B202307026146);Natural Science Foundation of Hainan Province,China(No.821QN1005)。

摘　　要：采用四氟硼酸(HBF4)与氢氟酸(HF)复合酸蚀刻和真空抽滤法制备Nb_(2)C MXene薄膜。利用扫描电子显微镜(SEM)、原子力显微镜(AFM)和X射线光电子能谱(XPS)等方法表征Nb_(2)C MXene的表面结构、性质和组成。通过体内和体外实验评价Nb_(2)C MXene薄膜的生物相容性。结果表明,Nb_(2)C MXene具有层状、微纳表面结构,表面富集大量羟基。相比于对照组,1mg/mL Nb_(2)C MXene培养的结膜成纤维细胞增殖率达到72.15%(p<0.05)。Nb_(2)C MXene的细胞毒性评估为0级(p>0.05),其溶血率为0.38%(<5%)。Nb_(2)C MXene薄膜降低兔眼组织的炎症和氧化应激反应,在不诱导纤维化的情况下促进伤口愈合,其微纳表面促进细胞的黏附和增殖,表现出良好的生物相容性。The Nb_(2)C MXene film was prepared by tetrafluoroboric acid(HBF4)and hydrofluoric acid(HF)etching and vacuum extraction.The surface structure,properties,and composition of Nb_(2)C MXene were characterized by scanning electron microscopy(SEM),atomic force microscopy(AFM),X-ray photoelectron spectroscopy(XPS),and other methods.The biocompatibility of Nb_(2)C MXene film was evaluated by in vivo and in vitro experiments.The results show the layered,micro−nano surface structure of Nb_(2)C MXene with abundant hydroxyl groups(—OH)on the surface.Compared with the control group,the proliferation rate of conjunctival fibroblasts cultured with 1 mg/mL Nb_(2)C MXene is 72.15%(p<0.05).The cytotoxicity of Nb_(2)C MXene is Grade 0(p>0.05),and the hemolysis rate is 0.38%(<5%).The oxidative stress and inflammatory responses in rabbit ocular tissues are inhibited by Nb_(2)C MXene film,which promotes wound healing without inducing fibrosis,and its micro−nano structure promotes cell adhesion and proliferation,demonstrating good biocompatibility.

关 键 词：Nb_(2)C MXene 生物相容性 氧化应激 细胞增殖 眼科应用 

分 类 号：TB383.2[一般工业技术—材料科学与工程] R318.08[医药卫生—生物医学工程]