干旱逆境下施用外源褪黑素对高粱生理调节的影响  

Effects of Exogenous Melatonin Application on Physiological Regulation of Sorghum under Drought Stress

作　　者：邹询 周宇飞[2] 李广[1] 王建忠[1] 王佳旭 张飞[1] ZOU Xun(Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning 110161)

机构地区：[1]辽宁省农业科学院,辽宁沈阳110161 [2]沈阳农业大学农学院,辽宁沈阳110186

出　　处：《园艺与种苗》2024年第4期84-88,110,共6页Horticulture & Seed

基　　金：农产品质量安全学科建设项目(2022DD113318);辽宁省藏粮于技重大专项(2023JH1/10200001);辽宁省农科院院绩效后补助项目(2022-HBZ-1008);沈阳市种业创新专项(22-318-2-15);辽宁省农业科学院基本科研业务费(基金类)项目(2021QN2011)。

摘　　要：[目的]研究外源褪黑素对高粱幼苗干旱胁迫下的生长影响和筛选适宜浓度。[方法]以高粱品种辽糯11为材料,采用15%PEG模拟干旱胁迫,设置0、60、120、180、240和300μmol/L等6个不同浓度梯度浸种,研究干旱胁迫下不同褪黑素浓度对高粱种子芽苗表型参数、渗透调节参数等指标的影响。[结果]不同褪黑素浓度处理下,渗透调节参数中游离氨基酸含量、脯氨酸含量、可溶性糖含量在180μmol/L褪黑素处理下含量最高,质膜透性在0~180μmol/L间不会受到损伤;抗氧化酶指标中,POD、SOD、CAT等活性指标在180μmol/L与300μmol/L达到最高,MDA含量与褪黑素浓度呈负相关,在180~300μmol/L处于较低水平。[结论]外源褪黑素180μmol/L的浓度处理对干旱逆境下高粱芽苗生长改善效果最佳。[Objective]To study the effects of exogenous melatonin on the growth of sorghum seedlings under drought stress and screen the appropriate concentration.[Method]The sorghum variety Liaonuo 11 was used as the material,and 15%PEG was used to simulate drought stress.Six different concentration gradients of 0,60,120,180,240 and 300μmol/L were set up to study the effects of different melatonin concentrations on the phenotypic parameters and osmotic adjustment parameters of sorghum seed buds and seedlings under drought stress.[Result]Under different melatonin concentrations,the contents of free amino acid,proline and soluble sugar in osmotic adjustment parameters were the highest under 180μmol/L melatonin treatment,and the plasma membrane permeability was not damaged between 0-180μmol/L.Among the antioxidant enzyme indexes,POD,SOD,CAT and other activity indexes reached the highest in 180μmol/L and 300μmol/L,MDA content was negatively correlated with melatonin concentration,and was at a low level from 180μmol/L to 300μmol/L.[Conclusion]The concentration of exogenous melatonin 180μmol/L had the best effect on the growth of sorghum seedlings under drought stress.

关 键 词：褪黑素 高粱 干旱 抗氧化指标 渗透调节 

分 类 号：S514[农业科学—作物学]