猪轮状病毒OSU株对乳鼠的致病性  

Pathogenicity of Porcine Rotavirus OSU Strain on Neonatal Mice

作　　者：桑卡娜 杨绒娟 胡惠君 卞愧 徐倩 褚何红 周德刚 SANG Kana;YANG Rongjuan;HU Huijun;BIAN Kui;XU Qian;CHU Hehong;ZHOU Degang(Luoyang Huizhong Animal Medicine Co.,Ltd.,National Research Center for Veterinary Medicine,Luoyang 471003,China;Xinyang Shihe District Agriculture and Rural Affairs Bureau,Xinyang 464000,China;China Institute of Veterinary Drug Control,Beijing 100081,China)

机构地区：[1]洛阳惠中兽药有限公司,国家兽用药品工程技术研究中心,河南洛阳471003 [2]信阳市浉河区农业农村局,河南信阳464000 [3]中国兽医药品监察所,北京海淀100081

出　　处：《中国兽医杂志》2024年第6期47-53,共7页Chinese Journal of Veterinary Medicine

摘　　要：为建立猪轮状病毒(PoRV)OSU株感染乳鼠致腹泻模型,研究PoRV对乳鼠的致病性,本试验选用40只4日龄昆明乳鼠,将其随机分为3个攻毒组和1个空白对照组,攻毒组乳鼠经口灌胃不同剂量的PoRV培养液,分别为攻毒组1(50μL)、攻毒组2(100μL)和攻毒组3(150μL),病毒滴度为10~(5.2)TCID_(50)/100μL,空白对照组按同样的方法灌服细胞培养液。在感染后不同时间点观察乳鼠的临床症状、腹泻和死亡情况、解剖症状,采用苏木精-伊红(H.E.)染色进行肠道组织病理学检查,反转录聚合酶链式反应(RT-PCR)检测乳鼠肠内容物中PoRV含量。结果显示,乳鼠在接种PoRV后24 h出现明显腹泻症状,攻毒后3~4 d时发病最严重,4 d时乳鼠的腹泻率介于62.5%~100%,7 d时恢复正常,乳鼠的死亡率介于44.4%~90.0%。与空白对照组比,攻毒组小鼠在给予PoRV后,肠道出现明显的病理变化,主要表现为水肿和充血、肠上皮完整性破坏。RT-PCR检测结果显示,攻毒组部分乳鼠的肠内容物中检出PoRV。本试验利用PoRV OSU株成功建立了乳鼠腹泻模型,阐明了其致病特征。To establish a mouse diarrhea model caused by porcine rotavirus(PoRV)OSU strain and investigate its pathogenicity in neonatal mice,forty 4-day-old Kunming mice were used in this study.The mice were randomly divided into three infected groups and one control group.The infected groups were orally administered different doses of PoRV culture fluid,with doses of Infection group 1(50μL),Infection group 2(100μL),and Infection group 3(150μL),with a viral titer of 105.2 TCID 50/100μL.The Control group was orally administered cell culture fluid using the same method.Clinical symptoms,diarrhea,mortality,and autopsy findings of the neonatal mice were observed at different time points after infection.Histopathological examination of intestinal tissues was conducted using hematoxylin eosin(H.E.)staining,and the PoRV content in the intestinal contents was detected using reverse transcription-polymerase chain reaction(RT-PCR).The results showed that neonatal mice exhibited significant diarrhea symptoms at 24 hours after PoRV inoculation.The most severe symptoms occurred on days 3 to 4 post-infection,with diarrhea rates ranging from 62.5%to 100%.By day 7,the neonatal mice had recovered to normal,with mortality rates ranging from 44.4%to 90.0%.Compared to the Control group,mice in the infected groups exhibited pronounced pathological changes in the intestines,mainly characterized by edema,congestion,and disruption of intestinal epithelial integrity.RT-PCR results showed the presence of PoRV in the intestinal contents of some mice in the infected groups.This study successfully established a mouse diarrhea model using the PoRV OSU strain and elucidated its pathogenic characteristics.

关 键 词：猪轮状病毒 乳鼠 腹泻 动物模型 反转录聚合酶链式反应(RT-PCR) 

分 类 号：S852.651[农业科学—基础兽医学]