地源砷对微生物多样性及群落结构的影响  

Microbial diversity and community structure under ground-source arsenic stress

作　　者：杨静茹 吕树萍 李巧[1,2] 宿彦鹏 陶洪飞 姜有为[1,2] 马合木江·艾合买提 YANG Jingru;LÜShuping;LI Qiao;SU Yanpeng;TAO Hongfei;JIANG Youwei;AIHEMAITI Mahemujiang(College of Hydraulic and Civil Engineering,Xinjiang Agricultural University,Urumqi 830052,China;Xinjiang Key Laboratory of Hydraulic Engineering Security and Water Disasters Prevention,Urumqi 830052,China;Water Resources Bureau of Usu City in Xinjiang,Usu 834700,China;Water Resources Bureau of Guanghe County,Linxia Hui Autonomous Prefecture in Gansu,Linxia 731300,China)

机构地区：[1]新疆农业大学水利与土木工程学院,乌鲁木齐830052 [2]新疆水利工程安全与水灾害防治重点实验室,乌鲁木齐830052 [3]新疆乌苏市水利局,乌苏834700 [4]甘肃省临夏回族自治州广河县水务局,临夏731300

出　　处：《应用与环境生物学报》2024年第2期309-316,共8页Chinese Journal of Applied and Environmental Biology

基　　金：新疆维吾尔自治区自然科学基金青年科学基金项目(2022D01B86);国家自然科学基金项目(41762018);新疆维吾尔自治区高校基本科研业务费科研项目(XJEDU2022J008);新疆水利工程安全与水灾害防治重点实验室研究项目(ZDSYS-YJS-2022-20)资助。

摘　　要：微生物的群落结构特征对地源砷的释放及富集过程具有重要意义,地源砷是微生物群落结构变化的关键因素,研究微生物对砷的响应有助于认识微生物对不同砷浓度的适应与演变机理.通过野外调查、高通量测序、微生物分析的方法研究奎屯河流域不同浓度地源砷(S1:低砷浓度组;S2:较高砷浓度组)对微生物的多样性及群落结构的影响.结果发现,地源砷会导致微生物多样性和群落结构发生变化.微生物多样性分析发现,地源砷浓度的增加使S2组的Ace、Simpson指数的平均值分别降低87.2和0.04,Simpson指数呈先增加后减少的趋势.S1组和S2组之间的微生物组成具有异同性.相似性为S1组与S2组有3个相同的优势菌群,分别为Arothrobacter、Acinetobacter和Novosphingobium.然而S2组中优势菌群的占比分别比S1组大8.01%、1.32%和3.21%.db-RDA和网络分析发现砷和采样深度对微生物群落有较大影响,S2组的微生物种间相互作用较弱.本研究表明地源砷浓度升高会使微生物群落向高耐性、高稳定性、单一性发展.(图6表1参44)The role of microbial community structure characteristics in the release and enrichment of groundsource arsenic(As)is highly important,with ground-source As being a major factor in altering the microbial community structure.Studying the response of microorganisms to As can help us understand the mechanisms of adaptation and evolution of microbes to different As concentrations.This study investigated the impact of varying ground-source As concentrations(S1 group with low As concentration and S2 group with higher As concentration)on the microbial diversity and community structure of the Kuitun River Basin,utilizing field investigation,high-throughput sequencing,and microbial analysis.These results indicated that ground-source As could alter microbial diversity and community structure.Diversity analysis revealed an increased groundsource As concentration,which reduced the mean values of the Ace and Simpson indices in the S2 group by 87.2 and 0.04,respectively.Simpson’s index initially increased and then decreased.The microbial compositions of the S1 and S2 groups demonstrated similarities and differences.The S1 group had three dominant bacteria that were identical to the S2 group,namely Arthrobacter,Acinetobacter,and Novosphingobium.However,the corresponding proportions of dominant bacteria in the S2 group were 8.01%,1.32%,and 3.21%higher than those in the S1 group.Distance-based redundancy and network analyses demonstrated that As and sampling depth significantly influenced the microbial community and that the interaction between microbial species in the S2 group was weak.These results show that an increased ground-source As concentration leads to high tolerance,high stability,and singleness of the microbial community.

关 键 词：地源砷 微生物 网络分析 奎屯河流域 

分 类 号：Q94[生物学—植物学]