克拉玛依市蚊虫携带菌群鉴定及研究  

Identification and study of mosquito-borne flora in Karamay City

作　　者：黄雨莲 玛伊热·海肉拉 杜菲 李昱辰 赵立萍 陈芋璋 郭万海 张荣 HUANG Yulian;Mayire·Hairoula;DU Fei;LI Yuchen;ZHAO Liping;CHEN Yuzhang;GUO Wanhai;ZHANG Rong(School of Basic Medical Sciences,Xinjiang Second Medical College,Karamay,Xinjiang Uygur Autonomous Region 834000,China;不详)

机构地区：[1]新疆第二医学院基础医学院,新疆维吾尔自治区克拉玛依834000 [2]克拉玛依市中心医院

出　　处：《医学动物防制》2024年第5期422-425,共4页Journal of Medical Pest Control

基　　金：2021年自治区级大学生创新创业训练计划项目(S202113560008);新疆维吾尔自治区自然科学基金资助项目(2022D01B152);克拉玛依市科技计划项目-创新环境建设计划(创新人才)(20212022hjcxrc0045)。

摘　　要：目的培养并鉴定克拉玛依市蚊虫携带的细菌,对鉴定结果进行分析,为蚊虫的研究与防治提供资料与数据支持。方法2021年9月—2022年6月,用血琼脂平板对蚊虫携带的细菌进行培养、分离与纯化,通过质谱仪鉴定菌落,辅以生化实验并查阅文献后进行分析。结果对新疆维吾尔自治区克拉玛依市随机抓取的蚊虫所携带的细菌进行鉴定,共得到两种菌,分别为革兰染色阴性、阿拉伯糖发酵试验和棉子糖发酵试验阳性,鉴定为深红沙雷菌;革兰染色阳性、动力试验阳性、卵黄培养基有乳白色浑浊环和甘露醇生化反应阴性,鉴定为蜡样芽孢杆菌。结论蚊虫可引起多种疾病如登革热、疟疾、丝虫病等。本实验得到的蜡样芽孢杆菌和深红沙雷菌均属于条件致病菌,可引起人体不同程度患病。致病性蜡样芽孢杆菌通过芽孢繁殖,包括食源性和血源性两类,部分食源性蜡样芽孢杆菌产肠毒素和呕吐毒素,引起腹泻和呕吐症状。致病性蜡样芽孢杆菌对β-内酰胺类抗生素等耐药,从而引起临床隐患。深红沙雷菌属于沙雷菌属,其临床菌株易对常用抗生素产耐药性,同时会引起尿路感染等疾病。Objective To culture and identify the mosquito-borne flora in Karamay City,to analyze the identification results,and to provide information and data support for the research and control of mosquitoes.Methods From September 2021 to June 2022,mosquito-borne flora were cultured,isolated and purified by blood agar plates.Colonies were identified by mass spectrometer,supplemented by biochemical experiments and analyzed after reviewing literature.Results Mosquito-borne flora were identified by random sampling in Karamay City,Xinjiang Uygur Autonomous Region.Two kinds of bacteria were obtained,which were gram staining negative,arabinose fermentation test and raffinose fermentation test positive,respectively,and were identified as Serratia rubidaea.Positive gram staining,positive dynamic test,milky white turbid ring in yolk medium and negative mannitol biochemical reaction were identified as Bacillus cereus.Conclusion Mosquitoes cause a variety of diseases,such as dengue fever,malaria and filariasis.Both Bacillus cereus and Serratia rubidaea obtained in this experiment are conditioned pathogens and cause diseases in human beings to different degrees.Pathogenic Bacillus cereus reproduces by spores,both foodborne and bloodborne.Partial foodborne Bacillus cereus produces enterotoxin and vomitoxin,which causes diarrhoea and vomiting symptoms.Pathogenic Bacillus cereus has drug resistance toβ-lactam antibiotics,which leads to a clinical hidden trouble.Serratia rubidaea belongs to the genus Serratia.Its clinical strains are susceptible to developing resistance to common antibiotics,as well as causing disease as urinary tract infection etcetera.

关 键 词：蚊虫 菌群 腊样芽孢杆菌 沙雷菌属 条件致病菌 耐药性 研究 

分 类 号：R184.3[医药卫生—流行病学]