基于数字全息的光场动态显微定量探测系统  

Development of a Dynamic Microscopy Quantitative Detection System Based on Digital Holography

作　　者：卞可歆 毕钰莹 任双文 田璐妤 BIAN Kexin;BI Yuying;REN Shuangwen

机构地区：[1]南京师范大学,南京210000

出　　处：《科技创新与应用》2024年第18期63-66,共4页Technology Innovation and Application

摘　　要：该文提出一种新型检测显微样本光学参数的方法,将显微数字全息术与相位解包裹的方法相结合,搭建实验光路并编写相关算法,从而由数字全息图像获得光场物体振幅及相位信息,利用折射率计算可以得到微米级样本的真实形状与厚度。该文采用光纤的纤芯验证试验系统的准确性,将洋葱鳞片表皮细胞作为实验对象,结果证实该实验方法的有效性以及可行性。This paper proposes a new method for detecting optical parameters of microscopic samples.It combines digital holography with phase unwrapping techniques,establishes an experimental optical setup,and develops relevant algorithms.Consequently,it extracts both the amplitude and phase information of the object's optical field from digital holographic images.By calculating the refractive index,it provides accurate measurements of the real shape and thickness of micrometer-scale samples.The accuracy of the proposed experimental system is validated using the core of an optical fiber,and the effectiveness and feasibility of the experimental method are demonstrated using onion epidermal cells.

关 键 词：显微定量测量 相位解包裹 折射率 光场 数字全息 

分 类 号：O438.1[机械工程—光学工程]