瞿麦(石竹)配方颗粒质量与饮片特征图谱的相关性  

Correlation between the quality of Dianthi Herb(Dianthus chinensis L.)formula granule and characteristic map of decoction pieces

作　　者：杜微波 陈敬然 万莹莹 李雪 马智玲 张志强 沈建梅 DU Weibo;CHEN Jingran;WAN Yingying;LI Xue;MA Zhiling;ZHANG Zhiqiang;SHEN Jianmei(Beijing Tcmages Pharmaceutical Company Limited,Beijing 101301,China;Traditional Chinese Medicine Formula Granule Key Technology National United Engineering Research Center,Beijing 101301,China;Beijing Chinese Medicine Formula Granule Engineering Technology Research Center,Beijing 101301,China)

机构地区：[1]北京康仁堂药业有限公司,北京101301 [2]中药配方颗粒关键技术国家地方联合工程研究中心,北京101301 [3]北京市中药配方颗粒工程技术研究中心,北京101301

出　　处：《河北工业科技》2024年第3期169-174,共6页Hebei Journal of Industrial Science and Technology

摘　　要：为了建立基于饮片特征图谱预测瞿麦(石竹)配方颗粒质量指标的方法,探究瞿麦(石竹)配方颗粒质量与饮片特征图谱的相关性。利用特征图谱对瞿麦(石竹)原料中不同药用部位的化学成分进行表征,明确差异峰,统计15批饮片特征图谱中差异峰占总特征峰的峰面积比值(α值);同时依据国药瞿麦(石竹)配方颗粒标准对制备的15批饮片瞿麦(石竹)配方颗粒总黄酮含量、出膏率、浸出物含量等指标进行测定;采用Pearson相关性分析方法对饮片特征图谱中的α值与配方颗粒的总黄酮含量、出膏率、浸出物含量进行相关性分析,建立回归模型,并采用具有生产规模的6批瞿麦(石竹)原料、配方颗粒质量参数进行验证。结果表明:王不留行黄酮苷峰和皂草苷峰在饮片中不同药用部位的特征图谱存在相对峰面积差异,15批饮片中二者峰面积总和的α值为0.45~0.83;通过Pearson相关性分析发现,瞿麦(石竹)配方颗粒的总黄酮含量、出膏率、浸出物含量与α值呈显著正相关(P<0.01),总黄酮含量与α值极强相关,出膏率、浸出物含量与α值强相关;依据相关性建立的3个回归模型,所得R2均大于0.7,模型拟合度较高,通过6批数据的验证发现预测值和实测值的相对标准偏差均小于15%,说明模型有较好的准确性。研究利用原料特征图谱中的α值能够初步预测所得瞿麦(石竹)配方颗粒的各指标范围,并可实现源头控制瞿麦(石竹)配方颗粒质量,保证所得配方颗粒质量的可控性。In order to establish the method of predicting the quality index of Dianthi Herb(Dianthus chinensis L.)formula granules based on the characteristic map of decoction pieces,the correlation between the quality of Dianthi Herb(Dianthus chinensis L.)formula granules and the characteristic map of decoction pieces was investigated.The chemical components of different medicinal parts in Dianthi Herb(Dianthus chinensis L.)raw materials were characterized by the characteristic atlas,the difference peak was defined,and the peak area ratio(α)of the difference peak to the total characteristic peak in the characteristic atlas of 15 batches of decoction pieces was calculated;At the same time,according to the national standard of Dianthi Herb(Dianthus chinensis L.)formula granules,the total flavonoids content,paste yield,extract and other indexes of Dianthi Herb(Dianthus chinensis L.)formula granules prepared by using the above 15 batches of decoction pieces were determined;Pearson correlation analysis method was used to analyze the correlation betweenαvalue in the characteristics of decoction pieces and the total flavonol content,paste yield and extract of formula granules,the corresponding regression model was established,and the quality parameters of six batches of Dianthi Herb(Dianthus chinensis L.)raw materials and formula particles with production scale were used for verification.The result shows that the relative peak area of vaccarin and saponarin in different medicinal parts in decoction pieces was different,and theαratio of the total peak area of the two peaks was 0.45~0.83 in 15 batches of decoction pieces;Through Pearson correlation analysis,it is found that the total flavonoids content,paste yield and extract are significantly positively correlated withαvalue(P<0.01),total flavonoids content is strongly correlated withαvalue,and paste rate and extract are strongly correlated withαvalue;According to the three regression models established according to the correlation,the R 2 obtained is all greater than 0.7,an

关 键 词：中药化学 饮片 配方颗粒 特征图谱 峰面积比值 相关性 可控性 

分 类 号：R283[医药卫生—中药学]