紫薇带芽茎段的离体培养及快速增殖  

In vitro Culture and Rapid Propagation of Stem Segments with Buds of Lagerstroemia indica

作　　者：闫娜 王春彦 胡红 何岭 YAN Na;WANG Chun-yan;HU Hong;HE Ling(Jinling Institute of Technology,Nanjing 210038,China)

机构地区：[1]金陵科技学院园艺园林学院,江苏南京210038

出　　处：《金陵科技学院学报》2024年第1期85-92,共8页Journal of Jinling Institute of Technology

基　　金：南京市绿化园林局科技项目(YLKJ202111JH);“十四五”江苏省重点学科园艺学建设基金(苏教研函[2022]2号);江苏省大学生创新训练计划项目(202213572100Y)。

摘　　要：以花瓣深粉红色的紫薇品种‘彩霞满天’(Lagerstroemia indica‘Caixia Mantian’)的半木质化带芽茎段为外植体,从消毒灭菌、初代培养基、增殖培养基等方面进行离体培养体系的优化。结果表明:各处理中紫薇茎段最佳消毒方案为先用75%酒精浸泡18 s,再用5%NaClO溶液浸泡8 min;腋芽茎段最佳启动培养基为1/2MS+6-BA 1.0 mg·L^(-1)+NAA 0.1 mg·L^(-1)+蔗糖20 g·L^(-1)+琼脂6.3 g·L^(-1),该培养基的平均诱导率达到45%,芽长势最好且叶色嫩绿;以基本培养基、6-BA浓度、KT浓度3个因素进行L9(33)正交试验,得出影响紫薇组培苗有效增殖系数的三因素主次程度为:6-BA>基本培养基>KT;对紫薇组培苗增殖及生长效果最好的培养基为MS+NAA 0.05 mg·L^(-1)+6-BA 2.0 mg·L^(-1)+KT 4.0 mg·L^(-1)+蔗糖30 g·L^(-1)+琼脂6.3 g·L^(-1)和WPM+NAA 0.05 mg·L^(-1)+6-BA 2.0 mg·L^(-1)+KT 4.0 mg·L^(-1)+蔗糖30 g·L^(-1)+琼脂6.3 g·L^(-1)。The semi-lignified stem segments with buds of Lagerstroemia indica‘Caixia Mantian’with dark pink petals were used as explants to optimize the in vitro culture system from the aspects of disinfection and sterilization,primary medium and proliferation medium.The results showed that the appropriate disinfection method for stem segments of L.indica was to first soak them in 75%alcohol for 18 s,and then soak them in 5%NaClO solution for 8 min.The optimal initiation medium for stem segment with axillary buds was 1/2MS+6-BA 1.0 mg·L^(-1)+NAA 0.1 mg·L^(-1)+sucrose 20 g·L^(-1)+agar 6.3 g·L^(-1),with an average induction rate reaching 45%,and then the buds had the best growth and the leaves was tender green in color.The L 9(33)orthogonal experiment design was carried out with three factors:basic medium,6-BA concentration and KT concentration.The main and secondary factors affecting the effective proliferation coefficient of L.indica tissue culture seedlings were found to be:6-BA>basic medium>KT.The culture media with the best effect on the proliferation and growth of the L.indica seedlings were MS+NAA 0.05 mg·L^(-1)+6-BA 2.0 mg·L^(-1)+KT 4.0 mg·L^(-1)+sucrose 30 g·L^(-1)+agar 6.3 g·L^(-1)and WPM+NAA 0.05 mg·L^(-1)+6-BA 2.0 mg·L^(-1)+KT 4.0 mg·L^(-1)+sucrose 30 g·L^(-1)+agar 6.3 g·L^(-1).

关 键 词：紫薇 茎段 离体培养 快速增殖 

分 类 号：S685[农业科学—观赏园艺]