基于UHPLC-Q-Exactive Orbitrap MS技术的退黄合剂化学成分快速表征  

Rapid analysis components of Tuihuang Mixture based on UHPLC-Q-Exactive Orbitrap MS

作　　者：陈桃灵 孟祥乐[2] 邹宇凤 王嘉怡 陈逸凡 李辉 CHEN Taoling;MENG Xiangle;ZOU Yufeng;WANG Jiayi;CHEN Yifan;LI Hui(Xiayi County Central Hospital,Shangqiu 476400,China;The First Affiliated Hospital of Henan University of TCM,Zhengzhou 450000,China;School of Pharmaceutical Sciences,Hunan University of Medicine,Huaihua 418000,China)

机构地区：[1]河南省夏邑县中心医院,河南商丘476400 [2]河南中医药大学第一附属医院,河南郑州450000 [3]湖南医药学院药学院,湖南怀化418000

出　　处：《广东药科大学学报》2024年第3期54-62,共9页Journal of Guangdong Pharmaceutical University

摘　　要：目的建立一种高效快速的超高效液相色谱串联四极杆轨道阱质谱(UHPLC-Q-Exactive Orbitrap MS)法,对退黄合剂的化学成分进行表征。方法采用Waters ACQUITY UPLC BEH C_(18)色谱柱(100 mm×2.1 mm,1.7μm),以流动相0.1%甲酸水溶液(A)-乙腈(B)进行梯度洗脱,流速为0.3 mL/min,进样量为2μL,柱温为40°C。质谱采用电喷雾离子源(ESI)负离子模式,质谱扫描范围为m/z 100~1500。结果通过对照品、数据库及参考文献共鉴定出退黄合剂中77种化学成分,其中包括黄酮类30种、多酚类18种、有机酸10种、萜类9种、蒽醌类9种、其他类1种。结论所建立的方法可高效、快速地鉴定退黄合剂的化学成分。Objective An effective and rapid analysis method by UHPLC-Q-Exactive Orbitrap MS was established to identify the components of Tuihuang Mixture.Methods The analysis was performed on a Waters ACQUITY UPLC BEH C_(18)(100 mm×2.1 mm,1.7μm)HPLC column.The mobile phase consisted of 0.1%formic acid(A)and acetonitrile(B)was used as gradient elute.The flow rate was 0.3 mL·min–1,column temperature was 40℃,and injection volume was 2μL.The ESI was operated in negative mode with the scanning range of m/z 100~1500.Results A total of 77 chemical components were identified by standards,database and references,including 30 flavonoid compounds,18 polyphenol compounds,10 organic acid compounds,9 terpenoids compounds,9 anthraquinone compounds,1 other constituent.Conclusion The established UHPLC-Q-Exactive Orbitrap MS technology can rapidly analyze and identify the components of Tuihuang Mixture.

关 键 词：退黄合剂 UHPLC-Q-Exactive Orbitrap MS 化学成分 快速表征 

分 类 号：R284.1[医药卫生—中药学]