邻近标记技术在蛋白质-蛋白质相互作用中的研究进展  

Research progress of proximity labeling in protein-protein interactions

作　　者：董娜 张文峰 DONG Na;ZHANG Wenfeng(School of Life Sciences and Biopharmaceutics,Guangdong Pharmaceutical University,Guangzhou 510006,China)

机构地区：[1]广东药科大学生命科学与生物制药学院,广东广州510006

出　　处：《广东药科大学学报》2024年第3期141-151,共11页Journal of Guangdong Pharmaceutical University

基　　金：广州市科技计划项目(202201010036);广东省基础与应用基础研究基金项目(2020A1515010889)。

摘　　要：蛋白邻近标记(proximity labeling,PL)技术是指通过融合特定的催化酶,实现对目标蛋白相互作用蛋白的原位标记,再采用质谱或测序分析鉴定标记蛋白。该技术能够在瞬时动态条件下精确识别蛋白质相互作用,是传统分析手段的重要补充,在蛋白质相互作用研究中具有广阔应用前景。本文介绍了各类PL酶的工作原理、分类及优缺点;论述了该技术在研究蛋白质与蛋白质相互作用中的应用;讨论了PL技术的优势、目前存在的问题和发展趋势,以期为该技术的应用提供参考。Proximity labeling(PL)technology refers to the in-situ labeling of protein-interacting proteins of interest by fusing specific catalytic enzymes,followed by mass spectrometry or sequencing analysis to identify the tagged proteins.This technique can accurately identify protein-protein interactions under transient dynamic condition,which is an important supplement to traditional analytical methods,and has a broad application prospect in the study of protein-protein interactions.In this paper,the working principle,classification,advantages and disadvantages of various PL enzymes were introduced,then the application of this technology in the study of protein-protein interactions was discussed,and finally the advantages,existing problems and development trends of adjacent labeling technology were discussed,in order to provide reference for the application of this technology.

关 键 词：蛋白邻近标记 蛋白质-蛋白质相互作用 工具酶 研究进展 

分 类 号：Q51[生物学—生物化学]