重复电针抑制前扣带回皮层腺苷酸环化酶1治疗神经病理性痛的研究  

Repeat Electroacupuncture Alleviates Neuropathic Pain Behaviors via Inhibiting Anterior Cingulate Cortex Adenylyl Cyclase 1

作　　者：陈思雨 杨海红 巩固 Chen Siyu;Yang Haihong;Gong Gu(School of Clinical Medical Sciences,Southwest Medical University,Luzhou 646000,China;Department of Anesthesiology,General Hospital of Western Theater Command,Chengdu 610083,China)

机构地区：[1]西南医科大学临床医学院,泸州646000 [2]解放军西部战区总医院麻醉科,成都610083

出　　处：《成都医学院学报》2024年第3期405-410,共6页Journal of Chengdu Medical College

基　　金：四川省自然科学基金面上项目(No:2022NSFSC0672)。

摘　　要：目的 探讨重复电针(REA)对坐骨神经慢性压迫损伤(CCI)大鼠神经病理性疼痛影响的中枢神经机制。方法 通过痛行为学检测、全细胞膜片钳、蛋白质印迹技术等方法检测CCI大鼠痛行为变化、前扣带回(ACC)突触可塑性变化以及腺苷酸环化酶(AC1)对REA镇痛效应的影响。结果 与对照组相比,CCI大鼠机械缩足阈值和热敏缩足潜伏期明显降低。CCI大鼠ACC锥体神经元动作电位基强度、阈值、半宽明显降低,幅值增大;微小兴奋性突触后电流(mEPSC)幅值增大,α-氨基-3羟基-5甲基-4异恶唑丙酸受体(AMPAR)表达增多。REA 2周,CCI模型的机械缩足阈值和热敏缩足潜伏期增大,抑制CCI大鼠神经病理性痛,表现累积镇痛效应。AC1激动剂foskolin阻断REA对CCI大鼠神经病理性痛的抑制效应。结论 REA通过抑制ACC锥体神经元AC1信号通路,促进突触传递功能恢复,抑制神经病理性痛。Objective To investigate the mechanism of analgesic effect of repeat electroacupuncture(REA)on neuropathic pain(NP)of rats with chronic constriction injury of the sciatic nerve(CCI).Methods NP behaviors,and changes of synaptic transmission and adenylyl cyclase 1(AC1)in the anterior cingulate cortex(ACC)were tested by mechanical and thermal pain testing,whole-cell patch-clamp recording,and Western blot at different time points.Results Compared to the sham group,paw withdrawal threshold and paw withdrawal latency both decreased significantly in CCI rats(P<0.05).Rheobase,threshold,and half-width of the first action potential(AP)decreased and the amplitude of AP increased obviously in the ACC pyramidal neurons of CCI rats(P<0.05).The amplitude and frequency of ACC pyramidal neurons micro excitatory postsynaptic current(mEPSC)enhanced a lot accompanied by the increase ofα-Amino-3-hydroxy-5-methyl-4-isoxazole receptors(AMPARs)following CCI(P<0.05).After the application of REA for 2 weeks,paw withdrawal threshold and paw withdrawal latency both increased,enhanced excitability of ACC pyramidal neurons decreased(P<0.05).The antinociceptive effect of REA on NP in CCI rats was inhibited by AC1 agonist foskolin.Conclusion REA alleviates NP behaviors via inhibiting ACC pyramidal neurons AC1 signaling and normalizing excitability and synaptic transmission.

关 键 词：重复电针 前扣带回 腺苷酸环化酶1 神经病理性痛 

分 类 号：R441.1[医药卫生—诊断学]