探讨姜黄素通过NF-κB对鼻咽癌CNE-2Z细胞的生长抑制作用、诱导凋亡作用及其机制研究  

Effect of curcumin on the growth inhibition and apoptosis induction of nasopharyngeal carci⁃noma CNE-2Z cells through NF-κB and its mechanism

作　　者：程忠强[1] 袁润生 蒋成义[1] 王伟[1] 强化龙[1] Cheng Zhongqiang;Yuan Runsheng;Jiang Chengyi;Wang Wei;Qiang Hualong(The First Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233004)

机构地区：[1]蚌埠医学院第一附属医院,安徽蚌埠233004

出　　处：《山西中医药大学学报》2024年第3期256-260,共5页Journal of Shanxi University of Chinese Medicine

基　　金：蚌埠医学院自然重点课题(2021byzd070)。

摘　　要：目的:探讨姜黄素通过NF-κB对鼻咽癌CNE-2Z细胞增殖的影响。方法:MTT法测定姜黄素对鼻咽癌CNE-2Z细胞增殖的影响,采用流式细胞仪测定姜黄素不同浓度下的CNE-2Z细胞凋亡情况,Western Blot测定NF-κB蛋白的表达。结果:姜黄素能影响CNE-2Z细胞的NF-κB蛋白表达,使NF-κB蛋白表达水平降低,说明姜黄素对CNE-2Z的增殖和转移有一定的影响。姜黄素对CNE-2Z的作用效果受作用时间和浓度影响,其对CNE-2Z细胞24 h的IC50数值为(43.514~45.435)μmol/L,48 h的IC50数值为(8.366~13.11)μmol/L,72 h的IC50数值为(5.658~8.469)μmol/L。结论:姜黄素能够通过NF-κB蛋白的表达,来抑制鼻咽癌CNE-2Z细胞增殖与侵袭。姜黄素浓度越高,作用时间越长,CNE-2Z细胞增殖抑制效果越好。Objective:To investigate the effect of curcumin on the proliferation of nasopharyngeal carcinoma CNE-2Z cells through NF-κB.Methods:The effect of curcumin on the proliferation of nasopharyngeal carcinoma CNE-2Z cells was determined by MTT method.The apoptosis of CNE-2Z cells at different concentrations of curcumin was determined by flow cytometer.The expression of NF-κB protein was determined by Western Blot.Results:Curcumin could affect the expression of NF-κB protein in CNE-2Z cells,and reduce the expression level of NF-κB protein,which indicated that curcumin had a certain effect on the proliferation and transfer of CNE-2Z.The effect of curcumin on CNE-2Z cells was affected by the action time and concentration.The IC50 values of curcumin on CNE-2Z cells for 24 h were(43.514~45.435)μmol/L,the IC50 values for 48 h were(8.366~13.11)μmol/L,and the IC50 values for 72 h were(5.658~8.469)μmol/L.Conclusion:The higher the concentration of curcumin and the longer the action time,the better the inhibitory effect of CNE-2Z cell proliferation.Curcumin can inhibit the proliferation and invasion of nasopharyngeal carcinoma CNE-2Z cells by expression of NF-κB protein.The higher the concentration of curcumin and the longer the action time,the better the inhibitory effect of CNE-2Z cell proliferation.

关 键 词：姜黄素 NF-ΚB 鼻咽癌 CNE-2Z细胞 生长 凋亡 

分 类 号：R285[医药卫生—中药学]